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dc.contributor.advisorShuping, Zhangeng
dc.contributor.authorYang, Mingeng
dc.date.issued2018eng
dc.date.submitted2018 Springeng
dc.description.abstractThe increasing prevalence of antibiotic-resistance and lack of effective antibiotics pose a serious threat to animal health and public health. Host antimicrobial peptides (AMPs) show broad-spectrum antimicrobial activity against various microbes with low potential for resistance development, compared to conventional antibiotics, indicating great potentials as therapeutic agents. Despite such promise, several limitations hinder the application of AMPs in the clinic, including high manufacturing cost, cytotoxicity, and stability in physiological conditions. New strategies are needed to solve those problems for their application. Avian beta-defensins (AvBD) are small, cationic, antimicrobial peptides. The potential application of AvBDs as antibiotic alternatives against antibiotic-resistant and zoonotic bacterial pathogens has been the subject of interest. In the first study, the biological functions of two AvBDs, AvBD-6 and AvBD-12, were determined under various experimental conditions. The results showed that AvBD-6 (+7) was more potent than AvBD-12 (+1) against E. coli, S. Typhimurium, and S. aureus as well as clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing E. coli and K. pneumoniae. The antibacterial activity of AvBDs was greatly compromised under physiological salt concentrations. Both AvBDs demonstrated mild chemotactic property for chicken macrophages and AvBD-12, at relatively high concentrations, could also induce the migration of murine immature dendritic cells. The chemotactic property required the presence of chemokine receptor 2 (CCR2) on host cells and the conserved disulfide bridges of the peptides. The two AvBDs were nontoxic to CHO-K1, macrophages, or immature dendritic cells.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxiv, 176 pages : illustrationseng
dc.identifier.urihttps://hdl.handle.net/10355/68953
dc.identifier.urihttps://doi.org/10.32469/10355/68953eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccesseng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Licenseeng
dc.subject.FASTAntibiotics--Researcheng
dc.subject.FASTPeptide antibioticseng
dc.titleAvian [beta]-defensins as antimicrobial and immunomodulatory agentseng
dc.typeThesiseng
thesis.degree.disciplinePathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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