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dc.contributor.advisorChen, Zhen, 1958-eng
dc.contributor.authorGan, Yong, 1976-eng
dc.date.issued2008eng
dc.date.submitted2008 Springeng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on Feb. 23, 2010 ).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDr. Zhen Chen, Dissertation Supervisor.eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionPh. D. University of Missouri--Columbia 2008.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Civil engineering.eng
dc.description.abstractTo develop a piezo intracytoplasmic sperm injection (piezo-ICSI) procedure without using mercury, the real physics behind the zona piercing must be understood. An effort has been made to formulate a three-dimensional model for fluid-membrane interaction with the use of the Material Point Method (MPM) so that the mouse zona piercing process could be better simulated for the piezo-ICSI procedure. It is found from the MPM simulations that the lateral vibration of the pipette leads to the noticeable oocyte deformation and the occurrence of the zona failure but no evident deformation and zona failure result from the longitudinal oscillation of the pipette. This outcome confirms the claim that the lateral vibration of the injection pipette plays a key role in the piercing process, and disproves the argument that the axial displacement of the pipette pierces the zona. The lateral vibration of the injection pipette is also analyzed using the finite element method (FEM) to further investigate the role of mercury in the zona piercing. It is shown that the employment of fluids generally reduces the vibration amplitude of the pipette tip and the amplitude decreases with the increase of fluid density. Moreover, smaller vibration amplitude could be observed as the pulse duration becomes shorter. It can be concluded that the effect of mercury on the piezo-ICSI might yield less oocyte damage due to the reduced amplitude of the pipette tip, and that the development of a piezo-ICSI procedure without using mercury is possible by changing the dynamic characteristics of the injection micropipette and the piezo-drill controller parameters.eng
dc.format.extentxxi, 142 pageseng
dc.identifier.oclc608543912eng
dc.identifier.urihttps://hdl.handle.net/10355/7111
dc.identifier.urihttps://doi.org/10.32469/10355/7111eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshPiezoelectric deviceseng
dc.subject.lcshSperm-ovum interactionseng
dc.subject.lcshZona pellucidaeng
dc.subject.lcshGenetic engineeringeng
dc.titleModel-based simulations of the piercing process in piezo-ICSIeng
dc.typeThesiseng
thesis.degree.disciplineCivil engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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