Characterizing Babesia genes for candidate vaccine peptides [abstract]

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Characterizing Babesia genes for candidate vaccine peptides [abstract]

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/712

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dc.contributor.author Williams, Rachel en
dc.date.accessioned 2009-03-18T18:46:35Z en
dc.date.available 2009-03-18T18:46:35Z en
dc.date.issued 2004 en
dc.identifier.uri http://hdl.handle.net/10355/712 en
dc.description Abstract only available en
dc.description Faculty Mentor: Dr. George Smith, Biological Sciences en
dc.description.abstract Babesiosis is a tropical cattle disease caused by the parasite Babesia bovis. This parasite infects red blood cells in cattle in much the same way as the malaria parasite Plasmodium falciparum infects red blood cells in humans. It also shares other characteristics that make babesiosis a good model disease for malaria. Using a phage-display strategy, certain peptides have previously been selected which could serve as components in a vaccine for babesiosis. The main goals of this project are to determine if the selected peptides are “natural” peptides, that is, parts of actual parasite proteins; and to gather available information about the function of those proteins. To accomplish this, we probed a B. bovis cDNA library with the coding sequences of selected phage-displayed peptides. The sequenced cDNA clones were used to determine if the phage-displayed peptide sequence matched the open reading frame of the cDNA clones. These cDNA clones were further used to learn about our peptides' function and location in B. bovis by comparison of the cDNA sequence to that of homologous proteins and known motifs. So far we have characterized clones from 24 different probes. Out of those clones 10 showed homology to known or hypothetical proteins, and 5 are putative membrane proteins. Membraneassociated proteins and other proteins that are expressed on the outer side of the parasite fit the mold of good vaccine components. en
dc.description.sponsorship Life Sciences Undergraduate Research Opportunity Program; National Institutes of Health en
dc.language.iso en_US en
dc.publisher University of Missouri--Columbia. Office of Undergraduate Research en
dc.relation.ispartof 2004 Undergraduate Research and Creative Achievements Forum (MU) en
dc.subject Babesia bovis (parasite) en
dc.subject malaria en
dc.subject phage-display strategy en
dc.subject peptides en
dc.title Characterizing Babesia genes for candidate vaccine peptides [abstract] en
dc.type Abstract en
dc.contributor.meetingname Undergraduate Research and Creative Achievements Forum (2004 : University of Missouri--Columbia) en
dc.contributor.corporatename University of Missouri-Columbia. Office of Undergraduate Research en
dc.relation.ispartofcommunity University of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forum


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