[-] Show simple item record

dc.contributor.authorAlbert, P.S.eng
dc.contributor.authorZhang, T.eng
dc.contributor.authorSemrau, K.eng
dc.contributor.authorRouillard, J.-M.eng
dc.contributor.authorKao, Y.-H.eng
dc.contributor.authorWang, C.-J.R.eng
dc.contributor.authorDanilova, T.V.eng
dc.contributor.authorJiang, J.eng
dc.contributor.authorBirchler, J.A.eng
dc.contributor.deptlabBiological Scienceeng
dc.date.issued2019eng
dc.description.abstractWhole-chromosome painting probes were developed for each of the 10 chromosomes of maize by producing amplifiable libraries of unique sequences of oligonucleotides that can generate labeled probes through transcription reactions. These paints allow identification of individual homologous chromosomes for many applications as demonstrated in somatic root tip metaphase cells, in the pachytene stage of meiosis, and in interphase nuclei. Several chromosomal aberrations were examined as proof of concept for study of various rearrangements using probes that cover the entire chromosome and that label diverse varieties. The relationship of the supernumerary B chromosome and the normal chromosomes was examined with the finding that there is no detectable homology between any of the normal A chromosomes and the B chromosome. Combined with other chromosome-labeling techniques, a complete set of whole-chromosome oligonucleotide paints lays the foundation for future studies of the structure, organization, and evolution of genomes.eng
dc.identifier10.1073/pnas.1813957116eng
dc.identifier.urihttps://hdl.handle.net/10355/75211
dc.identifier.urihttps://dx.doi.org/10.1073/pnas.1813957116eng
dc.languageEnglisheng
dc.publisherNational Academy of Scienceseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.eng
dc.rights.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0eng
dc.sourceProceedings of the National Academy of Sciences of the United States of Americaeng
dc.sourceAlbert, P.S., Zhang, T., Semrau, K., Rouillard, J.-M., Kao, Y.-H., Wang, C.-J.R., Danilova, T.V., Jiang, J., Birchler, J.A.. (2019). Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships. Proceedings of the National Academy of Sciences of the United States of America, 116(5). 10.1073/pnas.1813957116eng
dc.subjectB chromosome ; Chromosomal aberrations ; Chromosome painting ; Karyotype ; Oligo-FISHeng
dc.titleWhole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationshipseng
dc.typeArticleeng


Files in this item

[PDF]

This item appears in the following Collection(s)

[-] Show simple item record