dc.contributor.author | Li, Nancy | eng |
dc.contributor.author | Balaji, Boovaraghan | eng |
dc.contributor.author | Angel, Carlos Ariel, 1973- | eng |
dc.contributor.author | Cawly, John D., 1949- | eng |
dc.contributor.author | Schoelz, James E. (James Edgar), 1958- | eng |
dc.contributor.corporatename | University of Missouri-Columbia. Office of Undergraduate Research | eng |
dc.contributor.meetingname | Summer Undergraduate Research and Creative Achievements Forum (2006 : University of Missouri--Columbia) | eng |
dc.date.issued | 2006 | eng |
dc.description | Abstract only available | eng |
dc.description | Faculty Mentor: James Schoelz, Plant Pathology | eng |
dc.description.abstract | The N gene is a virus resistance gene found in the genome of Nicotiana glutinosa, which confers resistance to tobacco mosaic virus (TMV), among many other plant diseases. It is characteristic of a family of plant receptors that contain an N-terminal nucleotide-binding site (NBS) and a C-terminal leucine-rich repeat (LRR) which recognize and defend against attack by plant pathogens. Recently, a gene silencing assay has found that a second N. glutinosa resistance gene is responsible for targeting tombusviruses, such as tomato bushy stunt virus (TBSV), and is also closely related to the N gene. In order to identify and clone the TBSV resistance gene, NBS-LRR genes that are closely related to the N gene are being characterized. A previous PCR-based assay coupled with nucleotide sequencing indicated that the N-family of resistance genes consists of approximately 15 members. Currently, N homologs are being physically isolated from a N. glutinosa bacterial artificial chromosome (BAC) library. The N. glutinosa BAC library was probed with a portion of Exon1 of the N gene and to date, 13 BAC clones have been isolated. Portions of the N gene present on each of the BAC clones are being sequenced in an effort to further characterize the members of the N family of resistance genes. | eng |
dc.identifier.uri | http://hdl.handle.net/10355/763 | eng |
dc.publisher | University of Missouri--Columbia. Office of Undergraduate Research | eng |
dc.relation.ispartofcommunity | University of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forum | eng |
dc.source.uri | http://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=651 | eng |
dc.subject | N gene | eng |
dc.subject | Virus resistance | eng |
dc.subject | tobacco mosaic virus | eng |
dc.title | Isolation and characterization of the N family of resistance genes [abstract] | eng |
dc.type | Abstract | eng |