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dc.contributor.authorOstermeier, Austineng
dc.contributor.authorHardaway, John C., 1978-eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2006 : University of Missouri--Columbia)eng
dc.date.issued2006eng
dc.descriptionAbstract only availableeng
dc.descriptionFaculty Mentor: Habib Zaghouani, Molecular Microbiologyeng
dc.description.abstractEach year approximately 1.44 million deaths result from neonatal infection. Initial insights into the differences between the adult and neonatal immune systems were revealed by Sir Peter Medawar during his studies of organ transplantation. He discovered that skin grafts between different strains of mice, which normally result in a rejection of the graft between adult mice, could be successful if the recipient was initially transferred with splenic cells from the donor mouse during its neonatal stage of development. The phenomenon of neonatal tolerance has gone largely unexplained for nearly 50 years. Recent data from our lab supports a theoretical model for neonatal tolerance in which apoptosis of CD4+ T helper type I cells precludes rejection of the transplant. More specifically, we have demonstrated the involvement of the type II IL4 cytokine receptor in initiating this apoptotic signal. The IL13Rα1 cytokine receptor subunit is one component of this heterodimeric receptor, creation of an antibody that functionally inactivates this subunit would therefore provide a useful reagent to carry out more articulate studies of the neonatal immune system. Utilizing a baculovirus vector encoding the extracellular domain of IL13Rα1, we have expressed and purified recombinant IL13Rα1 and have further immunized Armenian hamsters to initiate an antibody response. Soon, we will fuse the B cells derived from these animals with immortalized myeloma cells to generate a permanent cell line expressing an antibody specific for IL13Rα1.eng
dc.identifier.urihttp://hdl.handle.net/10355/805eng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=751eng
dc.subjectneonatal infectioneng
dc.subjectneonatal toleranceeng
dc.titleGenerating a neutralizing antibody against IL13R1? [abstract]eng
dc.typePresentationeng


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