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dc.contributor.authorKatwa, Pranitaeng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2005 : University of Missouri--Columbia)eng
dc.date.issued2005eng
dc.descriptionAbstract only availableeng
dc.descriptionFaculty Mentor: Doug Bowles, Veterinary Biomedical Scienceseng
dc.description.abstractMigration of smooth muscle cells from the media into the intima has been shown to play a significant role in atherosclerosis. Recent clinical trials of atherosclerosis indicate that testosterone (TST) protects against coronary heart disease, however, the role of TST in coronary smooth muscle (CSM) migration has not been determined. We tested the hypothesis that TST inhibits migration of CSM. Cultured subconfluent (dedifferentiated) and 6 day serum starved postconfluent (differentiated) porcine CSM from passages 2 to 6 were plated in 8μm pore, 96 well, chemotaxis plates (Millipore). Migration was stimulated by the addition of growth factor PDGF-BB (30ng/ml) for 4 hours in the presence and absence of 1nM, 20nM and 100nM TST. In both dedifferentiated and differentiated cells, TST inhibited migration at all concentrations in a dose-dependent manner (60, 85 and 90 % inhibition respectively). Chelerythrine (1μM), a PKC-specific inhibitor, completely blocked migration by TST. In differentiated cells, 18 hour pretreatment with TST showed similar inhibition. Our data demonstrate that TST inhibits CSM migration through a PKC-dependent mechanism. Supported by HL071574 and NASA.eng
dc.description.sponsorshipNASA; National Institute of Healtheng
dc.identifier.urihttp://hdl.handle.net/10355/807eng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2005 Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=291eng
dc.subjectatherosclerosiseng
dc.subjectcoronary heart diseaseeng
dc.subjectCultured subconfluenteng
dc.subjectstarved postconfluenteng
dc.titleTestosterone Inhibits Migration of Porcine Coronary Smooth Muscle Cells via PKC [abstract]eng
dc.typeAbstracteng


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