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dc.contributor.advisorPrather, Randall S.en_US
dc.contributor.authorBauer, Bethany Kayen_US
dc.date.issued2010eng
dc.date.submitted2010 Springen_US
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 3, 2010).en_US
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.en_US
dc.descriptionThesis advisor: Dr. Randall Prather.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionM.S. University of Missouri--Columbia 2010.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Animal sciences.en_US
dc.description.abstractIn vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos cultured in vitro. Gilts were artificially inseminated and on Day 2 one oviduct and the tip of a uterine horn were flushed and the recovered embryos were cultured in PZM3 for four days. On Day 6 the gilts were euthanized and the contra-lateral horn was flushed to obtain in vivo derived embryos. Total RNA was extracted from 3 pools of 10 blastocysts from each treatment. First and second strand cDNA was synthesized and then sequenced using Illumina sequencing. The reads were aligned to a custom-built database designed to represent the known porcine "transcriptome". A total of 1,170 database members were different between the two groups (P[less than]0.05), and 588 of those were at least 2-fold different. Eleven transcripts were subjected to real-time PCR and each validated the sequencing. There was an overall decrease in both inner cell mass and trophectodermal cell numbers in embryos cultured in vitro, however, no difference in ICM:TE ratio was found. Interestingly, the transcript (SLC7A1) was higher in the in vitro cultured group. This difference disappeared after addition of arginine (0.36 mM) to the 4 day culture. In conclusion, Illumina sequencing and alignment to a custom "transcriptome" successfully identified a large number of genes that yield clues to the derivation of culture media.en_US
dc.format.extentxi, 88 pagesen_US
dc.identifier.merlinb77767317
dc.identifier.oclc646494863en_US
dc.identifier.otherBauerB-050410-T3267en_US
dc.identifier.urihttp://hdl.handle.net/10355/8098
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2010 Freely available theses (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2010 Theses
dc.subject.lcshSwine -- Embryosen_US
dc.subject.lcshSwine -- Breedingen_US
dc.subject.lcshFertilization in vitroen_US
dc.subject.lcshOvum implantationen_US
dc.subject.lcshGenetic transcriptionen_US
dc.subject.lcshNucleotide sequenceen_US
dc.titleTranscriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryosen_US
dc.typeThesisen_US
thesis.degree.disciplineAnimal scienceseng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMastersen_US
thesis.degree.nameM.S.en_US


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