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dc.contributor.advisorLucy, Matthew C. (Matthew Christian), 1960-eng
dc.contributor.authorGreen, Jacob Elijah Colten, 1984-eng
dc.date.issued2010eng
dc.date.submitted2010 Springeng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 22, 2010).eng
dc.descriptionThesis advisor: Dr. Matthew C. Lucy.eng
dc.descriptionM.S. University of Missouri--Columbia 2010.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Reproductive management is a major economic concern in the dairy industry. Long intervals between TAI increases the days open and decrease the 21 day pregnancy rate. A major factor that causes long intervals between TAI is pregnancy detection technique. Common methods of pregnancy detection are palpation per rectum and transrectal ultrasonography. Chemical pregnancy detection is uncommonly used in the dairy industry but has the potential to decrease the interval between TAI by reducing the days to pregnancy detection. Certain genes have greater transcription in response to interferon-T (IFNT). Measurement of mRNA for three genes [interferon-stimulated gene 15 kDa protein (Isg15), myxovirus resistance gene 2 (Mx2), and 2'-5' oligoadenylate synthetase 1 (Oas1)] that are up-regulated in response to IFNT was used to diagnose pregnancy in cows and heifers. This pregnancy diagnosis approach was used in a 21 day resynchronization program with similar results in pregnancy per AI (P/AI) as conventional programs. An enzyme-linked immunosorbent assay (ELISA) for pregnancy-associated glycoproteins (PAG) was highly accurate regardless of parity, breed, or stage of gestation ([greater than or equal to] 25 days). This PAG ELISA was used in a 28 day CIDR Ovsynch resynchronization protocol and compared with a 35 day CIDR Ovsynch resynchronization protocol that used transrectal ultrasonography for pregnancy diagnosis. Shortening the interval between inseminations by using a chemical method of pregnancy diagnosis improved reproductive rates in dairy cattle.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxvi, 134 pageseng
dc.identifier.merlinb79531246eng
dc.identifier.oclc650074378eng
dc.identifier.urihttps://hdl.handle.net/10355/8141
dc.identifier.urihttps://doi.org/10.32469/10355/8141eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.subject.lcshDairy cattle -- Breedingeng
dc.subject.lcshDairy cattle -- Pregnancyeng
dc.subject.lcshDairy cattle -- Artificial inseminationeng
dc.subject.lcshGlycoproteinseng
dc.subject.lcshEstruseng
dc.titleEfficacy of interferon-stimulated genes and pregnancy-associated glycoproteins for pregnancy diagnosis within an estrus resynchronization protocol for dairy cattleeng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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