Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2005 Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2005 Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Disruption of Arabidopsis thaliana dihydrolipoyl acetyltransferase gene expression using T-DNA and RNA interference [abstract]

    Matzek, Brett
    View/Open
    [PDF] Disruption of Arabidopsis thaliana.pdf (27.43Kb)
    Date
    2005
    Contributor
    University of Missouri-Columbia. Office of Undergraduate Research
    Format
    Abstract
    Metadata
    [+] Show full item record
    Abstract
    Arabidopsis thaliana is commonly used as a model organism for plant functional genomics research. The genome is relatively small and has been completely sequenced, the complete life cycle is relatively short, it is amenable to genetic manipulation, and is closely related to economically important crop plants. It is evident from the results of the A. thaliana genome sequencing project that many proteins are encoded by small multi-gene families. What is the reason for this apparent redundancy? One possibility is that the functions of the genes are vital for plant survival and the genome has accumulated "backup" copies, in case the primary gene is damaged. Another possibility is that genes that appear alike actually play some specialized role. For example, one gene might be expressed only in flowers while another is expressed in the leaves. The A. thaliana includes three genes for the important respiratory enzyme dihydrolipoyl acetyltransferase (E2). Two of the genes encode a protein with a single lipoyl domain, while the third has two lipoyl domains. A good way to study the function of multiple genes is to disrupt their expression. The most common method of gene disruption in plants uses what is called "T-DNA insertional mutagenesis." This method produces non-functional proteins. Another, relatively new, method uses RNA interference (RNAi) to prevent protein translation. RNAi uses a naturally occurring pathway to destroy the mRNA prior to translation of the protein. By using a combination of these two methods of molecular manipulation we will be closer to answering the question of why these genes exist in duplicate.
    URI
    http://hdl.handle.net/10355/822
    Part of
    2005 Undergraduate Research and Creative Achievements Forum (MU)
    Collections
    • 2005 Undergraduate Research and Creative Achievements Forum (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems