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    • Graduate School - MU Theses and Dissertations (MU)
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    • 2010 Dissertations (MU)
    • 2010 MU dissertations - Freely available online
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    Dicer interacts with the P-granule component GLH-1 : both localize to C. elegans nuclear pores and to stress-induced oocyte RNP granules

    Beshore, Erica Leigh, 1982-
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    Date
    2010
    Format
    Thesis
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    Abstract
    P granules are ribonucleoprotein complexes specific to the cytoplasmic side of the nuclear pores of C. elegans germ cells. While P granules are implicated in post-transcriptional control of maternally-transcribed mRNAs, their function remains elusive. Our laboratory is particularly interested in the P-granule component GLH-1 (Germline RNA Helicase-1). Through genetic studies our laboratory has shown that GLH-1 is essential for fertility; however, the biochemical function of the GLH complex is still unknown. With immunoprecipitations and GST-pulldowns, we report that GLH-1 and the riboendonuclease Dicer bind one another and their interaction is not RNA dependent. Both GLH-1 protein and mRNA levels are reduced in the dcr- 1(ok247) mutant background; conversely, a reduction of DCR-1 protein in the glh-1(gk100) deletion strain is also observed. Thus, in complex in the C. elegans germline, these two proteins seem interdependent. In addition, evidence indicates Dicer protein levels, like those of GLH-1, are regulated by proteosomal degradation and are much increased when the Jun N-terminal kinase KGB-1 is missing in the kgb-1(um3) null. In the adult C. elegans germline DCR-1 is located throughout the cytoplasm as well as at the inner nuclear pores of the germ cell nuclei, in close opposition to GLH-1. Under stress conditions in oocytes GLH-1 and DCR-1 both re-locate and recruit other components to large cytoplasmic RNP granules. We hypothesize the GLH-1/DCR-1 complex may function in the transport, deposition, or regulation of maternally-transcribed mRNAs perhaps with their associated miRNAs.
    URI
    https://hdl.handle.net/10355/8295
    https://doi.org/10.32469/10355/8295
    Degree
    Ph. D.
    Thesis Department
    Microbiology (Medicine) (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2010 MU dissertations - Freely available online
    • Molecular Microbiology and Immunology electronic theses and dissertations (MU)

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