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dc.contributor.authorDrake, Michael T.eng
dc.contributor.authorRiley, Lela K. (Lela Kay)eng
dc.contributor.authorLivingston, Robert Schaad, 1964-eng
dc.date.issued2008eng
dc.description.abstractAntibodies to rat theilovirus (RTV) have been detected in rats for many years because of their serologic crossreactivity with strains of Theiler murine encephalomyelitis virus (TMEV) of mice. Little information exists regarding this pathogen, yet it is among the most common viruses detected in serologic surveys of rats used in research. In the study reported here, a novel isolate of RTV, designated RTV1, was cultured from the feces of infected rats. The RTV1 genome contained 8094 nucleotides and had approximately 95% identity with another rat theilovirus, NSG910, and 73% identity with TMEV strains. In addition, the genome size of RTV1 was similar to those of TMEV strains but larger than that reported for NSG910. Oral inoculation of Sprague-Dawley (SD) and CD male rats (n = 10 each group) with RTV1 revealed that SD rats were more susceptible than CD rats to RTV1 infection. At 14 d postinoculation, 100% of SD rats shed virus in the feces, and 70% were positive for RTV serum antibodies. By 56 d postinoculation 30% of SD rats continued to have detectable virus in the feces, and 90% had seroconverted. In contrast, in inoculated CD rats RTV was detected only in the feces at 14 d postinoculation, at which time 40% of CD rats were fecal positive. By 56 d postinoculation only 20% of CD rats had detectable RTV serum antibodies. Our data provide additional sequence information regarding a rat-specific Cardiovirus and indicate that SD rats are more susceptible than CD rats to RTV1 infection.eng
dc.description.sponsorshipThis work was supported by funds from a National Institutes ofHealth Postdoctoral Training in Comparative Medicine grant (T32-RR07004) and the Research Animal Diagnostic Laboratory (RADIL).eng
dc.identifier.citationDrake, M.T., Riley, L.K., and Livingston, R.S. (2008). Differential Susceptibility of SD and CD Rats to a Novel Rat Theilovirus. Comparative Medicine, 58(5), 458-464.eng
dc.identifier.urihttp://hdl.handle.net/10355/8481eng
dc.languageEnglisheng
dc.publisherAmerican Association for Laboratory Animal Scienceeng
dc.relation.ispartofVeterinary Pathobiology publications (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. College of Veterinary Medicine. Department of Veterinary Pathobiologyeng
dc.source.harvestedPubMed Centraleng
dc.subjectRACEeng
dc.subjectrapid amplification of cDNA endseng
dc.subjectMHG viruseng
dc.subject.lcshRats -- Diseaseseng
dc.subject.lcshSprague Dawley ratseng
dc.subject.lcshImmunoglobulinseng
dc.titleDifferential Susceptibility of SD and CD Rats to a Novel Rat Theiloviruseng
dc.typeArticleeng


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