ELISAs Using Human Bocavirus VP2 Virus-Like Particles for Detection of Antibodies Against HBoV

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ELISAs Using Human Bocavirus VP2 Virus-Like Particles for Detection of Antibodies Against HBoV

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/8485

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dc.contributor.author Lin, Feng
dc.contributor.author Guan, Wuxiang
dc.contributor.author Yang, Ningmin
dc.contributor.author Pintel, David J.
dc.contributor.author Qui, Jianming
dc.date.accessioned 2010-09-13T16:16:40Z
dc.date.available 2010-09-13T16:16:40Z
dc.date.issued 2008-04
dc.identifier.citation Feng, L., Guan, W., Yang, N., Pintel, D. and Qui, J. (2008). ELISAs Using Human Bocavirus VP2 Virus-Like Particls for Detection of Antibodies Against HBoV. Journal of Virological Methods, 149(1), 110-117. en_US
dc.identifier.other doi:10.1016/j.jviromet.2007.12.016
dc.identifier.uri http://hdl.handle.net/10355/8485
dc.description.abstract Human bocavirus (HBoV) has been identified worldwide in children with lower respiratory tract infections with an incidence of approximately 2% −11%. The role of HBoV in pathogenesis, however, is largely unknown, and little is known about the epidemiology of the virus. To study the seroepidemiology of HBoV infection, the capsid protein was expressed in insect cells. Expression of the putative major capsid protein VP2 in insect cells led to the formation of virus-like particles exhibiting the typical icosahedral appearance of parvoviruses with a diameter of approximately 20 nm. The expressed particles were used to establish an ELISA method, and serum samples from groups of children of various ages in China were tested for IgG antibodies against HBoV. HBoV antibodies were detected in as high as 36% of healthy children under 9 years. Of children hospitalized with lower respiratory tract infections, 31% were seropositive, and all age groups of these children showed a significantly higher level of HBoV IgG antibody than their healthy counterparts. When divided into age cohorts, results showed that more than 48% of healthy children had seroconverted by age of 4. Thus, HBoV appears to be a common infection in children. The potential pathogenesis of this virus, especially its role in lower respiratory tract infections in children warrants further investigation. en_US
dc.language.iso en_US en_US
dc.publisher Journal of Virological Methods en_US
dc.subject respiratory illness en_US
dc.subject Human bocavirus en_US
dc.subject.lcsh Parvovirus infections en_US
dc.subject.lcsh Parvoviruses -- Epidemiology en_US
dc.subject.lcsh Pediatric respiratory diseases en_US
dc.subject.lcsh Enzyme-linked immunosorbent assay en_US
dc.title ELISAs Using Human Bocavirus VP2 Virus-Like Particles for Detection of Antibodies Against HBoV en_US
dc.title.alternative Enzyme-linked Immunosorbent Assays Using Human Bocavirus VP2 Virus-Like Particles for Detection of Antibodies Against Human Bocavirus en_US
dc.type Article en_US
dc.source.harvested PubMed Central web site en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. School of Medicine. Department of Molecular Microbiology and Immunology


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