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dc.contributor.authorJouvenet, Nolwenneng
dc.contributor.authorNeil, Stuart J.D.eng
dc.contributor.authorBess, Cameroneng
dc.contributor.authorJohnson, Marc C.eng
dc.contributor.authorVirgen, Cesar A.eng
dc.contributor.authorSimon, Sanford M.eng
dc.contributor.authorBieniasz, Paul D.eng
dc.date.issued2006-12eng
dc.descriptionIncludes black and white and color illustrations.eng
dc.description.abstractRecently proposed models that have gained wide acceptance posit that HIV-1 virion morphogenesis is initiated by targeting the major structural protein (Gag) to late endosomal membranes. Thereafter, late endosome-based secretory pathways are thought to deliver Gag or assembled virions to the plasma membrane (PM) and extracellular milieu. We present several findings that are inconsistent with this model. Specifically, we demonstrate that HIV-1 Gag is delivered to the PM, and virions are efficiently released into the extracellular medium, when late endosome motility is abolished. Furthermore, we show that HIV-1 virions are efficiently released when assembly is rationally targeted to the PM, but not when targeted to late endosomes. Recently synthesized Gag first accumulates and assembles at the PM, but a proportion is subsequently internalized via endocytosis or phagocytosis, thus accounting for observations of endosomal localization. We conclude that HIV-1 assembly is initiated and completed at the PM, and not at endosomal membranes.eng
dc.identifier.citationPlasma Membrane Is the Site of Productive HIV-1 Particle Assembly. PLOS Biology, 4(12), e435.eng
dc.identifier.otherDOI: 10. 1371/journal.pbio.0040435eng
dc.identifier.urihttp://hdl.handle.net/10355/8503eng
dc.publisherPLOS Biologyeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. School of Medicine. Department of Molecular Microbiology and Immunologyeng
dc.source.harvestedPubMed Central web siteeng
dc.subjecthuman immunodeficiency virus type Ieng
dc.subjectGag proteinseng
dc.subjectvirion structural proteineng
dc.subject.lcshHIV (Viruses)eng
dc.subject.lcshCell membraneseng
dc.subject.lcshEndosomeseng
dc.titlePlasma Membrane Is the Site of Productive HIV-1 Particle Assemblyeng
dc.typeArticleeng


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