Symbiotic Seed Germination and Mycorrhizae of Federally Threatened Platanthera praeclara (Orchidaceae)

MOspace/Manakin Repository

Breadcrumbs Navigation

Symbiotic Seed Germination and Mycorrhizae of Federally Threatened Platanthera praeclara (Orchidaceae)

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/8603

[+] show full item record


Title: Symbiotic Seed Germination and Mycorrhizae of Federally Threatened Platanthera praeclara (Orchidaceae)
Author: Sharma, Jyotsna; Zettler, Lawrence W.; Van Sambeek, J. W.; Ellersieck, Mark R.; Starbuck, Christopher J.
Keywords: terrestrial orchids
pollination
herbivory
erratic flowering
Date: 2003
Publisher: The American Midland Naturalist
Citation: The American Midland Naturalist 149(1):104-120. 2003.
Abstract: In vitro culture of mycotrophic leaf-bearing seedlings of federally threatened Platanthera praeclara Sheviak and Bowles, a terrestrial orchid native to the midwestern prairies, is reported for the first time. Symbiotic germination was evaluated to: (1) determine need for cold moist stratification to enhance seed germination and seedling development and (2) identify the mycobionts that support in vitro germination and development. Germination was improved by exposing seeds to both 4- and 6-mo stratification periods; whereas seeds without stratification failed to germinate in a pilot study. Pretreatment of seeds with 6 mo of stratification combined with inoculation with mycorrhizal fungus derived from a seedling (Ceratorhiza sp., UAMH 9847) supported development of higher stage protocorms and some leaf-bearing seedlings of P. praeclara. Protocorms with developing leaf primordia were also obtained by coinoculation with strains of Epulorhiza and Ceratorhiza. Fungi derived from mature P. praeclara plants failed to promote seedling development to advanced growth stages. Results indicate that in vitro development of P. praeclara is best supported when stratified seeds are cultured with fungi isolated from young seedlings.
URI: http://hdl.handle.net/10355/8603
Other Identifiers: doi: 10.1674/0003-0031(2003)149[0104:SSGAMO]2.0.CO;2

This item appears in the following Collection(s)

  • Plant Sciences publications (MU) [3]
    The items in this collection are the scholarly output of the faculty, staff, and students of the Division of Plant Sciences.

[+] show full item record