Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • College of Veterinary Medicine (MU)
    • Department of Veterinary Medicine and Surgery (MU)
    • Veterinary Medicine and Surgery publications and datasets (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • College of Veterinary Medicine (MU)
    • Department of Veterinary Medicine and Surgery (MU)
    • Veterinary Medicine and Surgery publications and datasets (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Acylation of the Lipooligosaccharide of Haemophilus influenzae and Colonization: an htrB Mutation Diminishes the Colonization of Human Airway Epithelial Cells

    Swords, W. Edward
    Chance, Deborah L.
    Cohn, Leah A.
    Shao, Jianqiang
    Apicella, Michael A.
    Smith, Arnold L.
    View/Open
    [PDF] AcylationLipooligosaccharideHaemophilusInfluenza.pdf (1010.Kb)
    Date
    2002
    Format
    Article
    Metadata
    [+] Show full item record
    Abstract
    Haemophilus influenzae is a commensal and opportunistic pathogen of the human airways. A number of surface molecules contribute to colonization of the airways by H. influenzae, such as adhesins, including structures found in the lipooligosaccharide (LOS). A human bronchiolar xenograft model was employed to investigate the host-bacterial interactions involved in the colonization of the airway by H. influenzae. Differential display was used to identify H. influenzae mRNA that reflect genes which were preferentially expressed in the xenograft compared to growth. Eleven mRNA fragments had consistent increased expression when the bacteria grew in xenografts. On sequencing these fragments, eight open reading frames were identified. Three of these had no match in the NCBI or the TIGR database, while an additional three were homologous to genes involved in heme or iron acquisition and utilization: two of the mRNAs encoded proteins homologous to enzymes involved in LOS biosynthesis: a heptosyl transferase (rfaF) involved in the synthesis of the LOS core and a ketodeoxyoctonate phosphate-dependent acyltransferase (htrB) that performs one of the late acylation reactions in lipid A synthesis. Inoculation of human bronchiolar xenografts revealed a significant reduction in colonization capacity by htrB mutants. In vitro, htrB mutants elicited lesser degrees of cytoskeletal rearrangement and less stimulation of host cell signaling with 16HBE14o- cells and decreased intracellular survival. These results implicate acylation of H. influenzae lipid A as playing a key role in the organisms' colonization of the normal airway.
    URI
    http://hdl.handle.net/10355/8607
    Part of
    Veterinary Medicine and Surgery publications (MU)
    Citation
    Infection and Immunity, August 2002, p. 4661-4668, Vol. 70, No. 8.
    Collections
    • Veterinary Medicine and Surgery publications and datasets (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems