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dc.contributor.authorSwords, W. Edwardeng
dc.contributor.authorChance, Deborah L.eng
dc.contributor.authorCohn, Leah A.eng
dc.contributor.authorShao, Jianqiangeng
dc.contributor.authorApicella, Michael A.eng
dc.contributor.authorSmith, Arnold L.eng
dc.date.issued2002eng
dc.description.abstractHaemophilus influenzae is a commensal and opportunistic pathogen of the human airways. A number of surface molecules contribute to colonization of the airways by H. influenzae, such as adhesins, including structures found in the lipooligosaccharide (LOS). A human bronchiolar xenograft model was employed to investigate the host-bacterial interactions involved in the colonization of the airway by H. influenzae. Differential display was used to identify H. influenzae mRNA that reflect genes which were preferentially expressed in the xenograft compared to growth. Eleven mRNA fragments had consistent increased expression when the bacteria grew in xenografts. On sequencing these fragments, eight open reading frames were identified. Three of these had no match in the NCBI or the TIGR database, while an additional three were homologous to genes involved in heme or iron acquisition and utilization: two of the mRNAs encoded proteins homologous to enzymes involved in LOS biosynthesis: a heptosyl transferase (rfaF) involved in the synthesis of the LOS core and a ketodeoxyoctonate phosphate-dependent acyltransferase (htrB) that performs one of the late acylation reactions in lipid A synthesis. Inoculation of human bronchiolar xenografts revealed a significant reduction in colonization capacity by htrB mutants. In vitro, htrB mutants elicited lesser degrees of cytoskeletal rearrangement and less stimulation of host cell signaling with 16HBE14o- cells and decreased intracellular survival. These results implicate acylation of H. influenzae lipid A as playing a key role in the organisms' colonization of the normal airway.eng
dc.identifier.citationInfection and Immunity, August 2002, p. 4661-4668, Vol. 70, No. 8.eng
dc.identifier.issn0019-9567/02eng
dc.identifier.otherDOI: 10.1128/IAI.70.8.4661-4668.2002eng
dc.identifier.urihttp://hdl.handle.net/10355/8607eng
dc.languageEnglisheng
dc.publisherAmerican Society for Microbiologyeng
dc.relation.ispartofVeterinary Medicine and Surgery publications (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. College of Veterinary Medicine. Department of Veterinary Medicine and Surgeryeng
dc.subjectairway infectionseng
dc.subjectNTHieng
dc.subjectinvasive diseaseeng
dc.subject.lcshHaemophilus influenzaeeng
dc.subject.lcshAcylationeng
dc.subject.lcshAirway (Medicine) -- Infectionseng
dc.subject.lcshHost-bacteria relationshipseng
dc.titleAcylation of the Lipooligosaccharide of Haemophilus influenzae and Colonization: an htrB Mutation Diminishes the Colonization of Human Airway Epithelial Cellseng
dc.typeArticleeng


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