Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2006 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Office of Undergraduate Research (MU)
    • Undergraduate Research and Creative Achievements Forum (MU)
    • 2006 Summer Undergraduate Research and Creative Achievements Forum (MU)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    From tissue liquidity to vascular printing [abstract]

    Saenz, Diego
    Norotte, Cyrille, 1983-
    Forgács, Gabor, 1949-
    View/Open
    [PDF] From tissue liquidity to vascular printing.pdf (15.35Kb)
    Date
    2006
    Contributor
    University of Missouri-Columbia. Office of Undergraduate Research
    Format
    Abstract
    Metadata
    [+] Show full item record
    Abstract
    Over the last decades, many tissues have been shown to share common properties with liquids: tissue fragments round up as liquid drops and fuse with similar kinetics of liquids. Also of interest is the phase separation phenomenon undergone by mixed cell populations of different adhesiveness mimicking the "breaking" of a dispersion or emulsion of two immiscible liquids. For a system to show such behavior it must (1) be composed of many subunits that (2) cohere while (3) being mobile. These are the defining characteristics of a liquid. In ordinary liquids the subunits are molecules and the mobility is Brownian. Rearranging cell populations also possess these same three properties that underlie liquid behavior, but their subunits are living cells whose mobility may be either active, driven by intracellular forces, or passive, pulled by external forces. In the present study, we used tissue-liquidity to generate microtissue droplets of smooth muscle cells, fibroblasts and endothelial cells that can serve as minimal building units for vascular tissue-engineering. Size-specific spheroids of SMCs and fibroblasts were first generated by cell aggregation in P96 well plates. As the extracellular matrix content of the vessel wall is essential for obtaining adequate mechanical properties in tissue-engineered vascular grafts, those tissue droplets were then matured over 4 weeks in the presence of ascorbic acid or TGF-1 and insulin, and then analyzed at various times by histology in order to assess collagen and elastin production. At last, straight and branching tubes of different diameters were achieved by the fusion of CHO cells tissue spheroids deposited by hand or by a bioprinter. In conclusion, we show that the understanding of the physical mechanisms underlying tissue organization can be easily applied in the field of tissue-engineering.
    URI
    http://hdl.handle.net/10355/862
    Collections
    • 2006 Summer Undergraduate Research and Creative Achievements Forum (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems