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    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2010 Dissertations (MU)
    • 2010 MU dissertations - Freely available online
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    Development of a biosensor for the detection of Helicobacter hepaticus utilizing multi-functioning immobilization platforms

    Memisevic, Jasenka, 1983-
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    [PDF] research.pdf (16.57Mb)
    Date
    2010
    Format
    Thesis
    Metadata
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    Abstract
    Optical biosensor development is a process involving the careful selection of the material for the immobilization platform, design of the detection mechanism and integration with optical and electronic equipment. The ability of biosensor to outperform the currently available technology, while reducing the cost and providing ease of use is of utmost importance. This work describes the research performed in effort to develop a high surface area material for biosensor immobilization, which improves the biosensor's function with the incorporation of fluorescence. Two silica-based nanoporous materials were evaluated, the nanoporous organosilicate (NPO) and fluorescent electrospun sol-gelnano/microfibers. The biosensor was also developed utilizing the immunosensing technique in a sandwich-based assay. The fluorescence based optical biosensor involves the use of organic fluorophores along with colloidal gold nanoparticles. When used as reporter molecules, the gold nanoparticles quench fluorescence of the fluorophores in the biosensor immobilization platform, thus signaling the presence of analytes. The results indicate that utilizing materials with high surface area to volume ratios, with embedded fluorescence, and the sandwich-based immunoassay provides a highly specific biosensor with a low level of detection.
    URI
    https://hdl.handle.net/10355/8867
    https://doi.org/10.32469/10355/8867
    Degree
    Ph. D.
    Thesis Department
    Biological engineering (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • Biological Engineering electronic theses and dissertations - Engineering (MU)
    • 2010 MU dissertations - Freely available online
    • Biological Engineering electronic theses and dissertations - CAFNR (MU)

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