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dc.contributor.advisorQuinn, Thomas P. (Thomas Patrick), 1961-en_US
dc.contributor.authorCalzada, Victoria
dc.contributor.authorZhang, Xiuli
dc.contributor.authorCabral, Pablo
dc.contributor.authorFernandez, Marcelo
dc.contributor.authorGambini, Juan Pablo
dc.date.issued2010-11eng
dc.description.abstractErbB-2 is a type 1 receptor tyrosine kinase over-expressed on ~30% of breast cancers and is an attractive target for the development of new diagnostic and therapeutic agents. The ErbB-2 avid peptide KCCYSL, originally identified from phage display, has shown promising imaging results in pre-clincial breast carcinoma animal models. In this study the peptide DOTA-GSGKCCYSL was radiolabeled with the beta-particle emitter lutetium-177 (177Lu) and examined for its therapeutic potential. The peptide DOTA-GSG-KCCYSL was synthesized using Fmoc solid phase peptide synthesis and purified to homogeneity. DOTA-GSG-KCCYSL was radiolabeled with Lu-177 and purified via high performance liquid chromatography. In vitro stability studies showed that the radiolabeled complex was stable to challenges from metal chelators and was stable to >60% in serum at 37˚C over 24 h. Cell binding of 177Lu-DOTA-GSG-KCCYSL to MB- 435 human breast carcinoma cells displayed saturable binding at 2 h. Log P studies indicated that the radiolabeled complex was very hydrophilic. Initial biodistribution studies in MB-435 xenografted mice showed modest tumor uptake of 1.82 % injected dose per gram (ID/g) 30 min post injection and 0.56 % ID/g at 1 h post injection. Additional biodistribution and imaging studies are underway to determine dosimetry and potential tumor therapeutic properties.en_US
dc.identifier.urihttp://hdl.handle.net/10355/9144
dc.relation.ispartof2010 Health Sciences Research Day (MU)en_US
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Health Sciences Research Day.
dc.subjectcancer treatmenten_US
dc.subjectmolecular characteristicsen_US
dc.title177Lu radiolabeled peptides for breast cancer therapy [abstract]en_US
dc.typeOtheren_US


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