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dc.contributor.advisorCalcutt, Michaeleng
dc.contributor.authorKent, Bethany N.eng
dc.date.issued2008eng
dc.date.submitted2008 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on November 19, 2010).eng
dc.descriptionVita.eng
dc.descriptionThesis advisor: Michael J. Calcutt.eng
dc.description"December 2008"eng
dc.descriptionPh. D. University of Missouri-Columbia 2008.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Horizontal gene transfer has played a major role in bacterial evolution, including in the genus Mycoplasma, which is comprised of some of the simplest and smallest free-living organisms. This dissertation focuses on the functional characteristics of two recently characterized putative mobile elements of the ruminant-infecting Mycoplasma mycoides cluster: a 3.4-kb cryptic plasmid, pMyBK1, of Mycoplasma yeatsii, and an approximately 24-kb member of the mycoplasmal family of Integrative, Conjugative Elements (ICE), ICEC, of Mycoplasma capricolum subsp. capricolum strain Kid. Vectors were constructed using pMyBK1 as a backbone and replication of vector derivatives in M. yeatsii identified the ORF required for survival of the plasmid. Showing the utility of the vector system, heterologous proteins were expressed in M. yeatsii from pMyBK1 derivatives. To determine which ORF of ICEC acts as an integrase, mini-ICEC derivatives were constructed. The right-hand terminal ORF, MCAP_0571, was shown to be the ORF required to integrate the mini-element into Mycoplasma capricolum subsp. capricolum strain GM262G.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxiii, 230 pageseng
dc.identifier.oclc682163079eng
dc.identifier.urihttps://hdl.handle.net/10355/9179
dc.identifier.urihttps://doi.org/10.32469/10355/9179eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.subject.meshMycoplasma mycoides -- geneticseng
dc.subject.meshGenetic Vectorseng
dc.subject.meshPlasmidseng
dc.subject.meshConjugation, Geneticeng
dc.titleFunctional characterization of two putative mobile elements of the 'Mycoplasma mycoides cluster'eng
dc.typeThesiseng
thesis.degree.disciplineMicrobiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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