dc.contributor.advisor | Reinero, Carol R. | eng |
dc.contributor.author | Sharp, Claire | eng |
dc.date.issued | 2010 | eng |
dc.date.submitted | 2010 Summer | eng |
dc.description | The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. | eng |
dc.description | Title from PDF of title page (University of Missouri--Columbia, viewed on November 9, 2010). | eng |
dc.description | Thesis advisor: Carol Reinero. | eng |
dc.description | "August 2010" | eng |
dc.description | M.S. University of Missouri-Columbia 2010. | eng |
dc.description.abstract | Introduction: Directed, effective therapies for feline sepsis are needed to reduce the high morbidity and mortality associated with this disease. Materials and Methods: We investigated the anti-endotoxin effects of PMB in a blinded, placebo controlled fashion, both ex vivo in a feline whole blood culture system and in vivo, using a low-dose endotoxin infusion in cats (2ug/kg/hr IV x 4 hours). Serial measures of systemic inflammation, and hemodynamic stability, were compared between groups. Results: Ex vivo, PMB significantly decreased LPS-induced TNF production from whole blood. In vivo, endotoxin infusion resulted in the development of fever, hypotension, leucopenia and increased TNF activity. Polymyxin B (1mg/kg over 30 minutes) treatment decreased peak plasma TNF activity (p<0.001) and increased white blood cell count (p=0.019), with no adverse effects. Conclusions: Polymyxin B administration resulted in decreased peak plasma TNF activity and increased white blood cell count in this feline model of endotoxemia, with no adverse effects. Given the apparent safety and anti-endotoxin effects of PMB in this endotoxemia model, a carefully designed, randomized, blinded, placebo controlled clinical trial evaluating the use of PMB in naturally occurring Gram negative feline sepsis should be considered. | eng |
dc.description.bibref | Includes bibliographical references. | eng |
dc.format.extent | xi, 55 pages | eng |
dc.identifier.merlin | b80699352 | eng |
dc.identifier.oclc | 680039178 | eng |
dc.identifier.uri | http://hdl.handle.net/10355/9298 | |
dc.identifier.uri | https://doi.org/10.32469/10355/9298 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2010 Theses | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | |
dc.subject.lcsh | Endotoxemia -- Treatment | eng |
dc.subject.lcsh | Cats -- Diseases -- Treatment | eng |
dc.subject.lcsh | Polymyxin -- Therapeutic use | eng |
dc.title | The antiendotoxin effects of polymyxin B in a feline model of endotoxemia | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Veterinary biomedical sciences (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Masters | eng |
thesis.degree.name | M.S. | eng |