The antiendotoxin effects of polymyxin B in a feline model of endotoxemia

MOspace/Manakin Repository

Breadcrumbs Navigation

The antiendotoxin effects of polymyxin B in a feline model of endotoxemia

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/9298

[-] show simple item record

dc.contributor.advisor Reinero, Carol R. en_US
dc.contributor.author Sharp, Claire en_US
dc.date.accessioned 2010-12-08T17:48:23Z
dc.date.available 2010-12-08T17:48:23Z
dc.date.issued 2010 en_US
dc.date.submitted 2010 Summer en_US
dc.identifier.other SharpC-082410-T24 en_US
dc.identifier.uri http://hdl.handle.net/10355/9298
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Title from PDF of title page (University of Missouri--Columbia, viewed on November 9, 2010). en_US
dc.description Includes bibliographical references en_US
dc.description Thesis advisor: Carol Reinero. en_US
dc.description "August 2010" en_US
dc.description M.S. University of Missouri-Columbia 2010. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Veterinary biomedical sciences. en_US
dc.description.abstract Introduction: Directed, effective therapies for feline sepsis are needed to reduce the high morbidity and mortality associated with this disease. Materials and Methods: We investigated the anti-endotoxin effects of PMB in a blinded, placebo controlled fashion, both ex vivo in a feline whole blood culture system and in vivo, using a low-dose endotoxin infusion in cats (2ug/kg/hr IV x 4 hours). Serial measures of systemic inflammation, and hemodynamic stability, were compared between groups. Results: Ex vivo, PMB significantly decreased LPS-induced TNF production from whole blood. In vivo, endotoxin infusion resulted in the development of fever, hypotension, leucopenia and increased TNF activity. Polymyxin B (1mg/kg over 30 minutes) treatment decreased peak plasma TNF activity (p<0.001) and increased white blood cell count (p=0.019), with no adverse effects. Conclusions: Polymyxin B administration resulted in decreased peak plasma TNF activity and increased white blood cell count in this feline model of endotoxemia, with no adverse effects. Given the apparent safety and anti-endotoxin effects of PMB in this endotoxemia model, a carefully designed, randomized, blinded, placebo controlled clinical trial evaluating the use of PMB in naturally occurring Gram negative feline sepsis should be considered. en_US
dc.format.extent xi, 55 pages en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.relation.ispartof 2010 Freely available theses (MU) en_US
dc.subject.lcsh Endotoxemia -- Treatment en_US
dc.subject.lcsh Cats -- Diseases -- Treatment en_US
dc.subject.lcsh Polymyxin -- Therapeutic use en_US
dc.title The antiendotoxin effects of polymyxin B in a feline model of endotoxemia en_US
dc.type Thesis en_US
thesis.degree.discipline Veterinary biomedical sciences en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.merlin b80699352
dc.identifier.oclc 680039178 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2010 Theses


This item appears in the following Collection(s)

[-] show simple item record