N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

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N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/9375

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Title: N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro
Author: Sharma, Ajay; Tandon, Ashish; Tovey, Jonathan; Gupta, Rangan, 1978-; Robertson, John David, 1960-; Fortune, Jennifer A.; Klibanov, Alexander M.; Cowden, John W.
Keywords: non-viral gene therapy
corneal diseases
Date: 2010-11-11
Abstract: Recently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo.
URI: http://hdl.handle.net/10355/9375

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