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    N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

    Sharma, Ajay
    Tandon, Ashish
    Tovey, Jonathan
    Gupta, Rangan, 1978-
    Robertson, John David, 1960-
    Fortune, Jennifer A.
    Klibanov, Alexander M.
    Cowden, John W.
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    [PDF] NPRatioPEI2-GNP-DNAComplex.pdf (271.8Kb)
    [PDF] NPRatioPEI2-GNP-DNAComplexPoster.pdf (1.073Mb)
    Date
    2010
    Contributor
    University of Missouri--Columbia. School of Medicine
    Format
    Other
    Metadata
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    Abstract
    Recently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo.
    URI
    http://hdl.handle.net/10355/9375
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
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