RT-PCR on magnetically sorted B-cell subsets from pediatric bone marrow [abstract]

Abstract

Acute lymphoblastic leukemia (ALL) is characterized by the uncontrolled proliferation of lymphoblasts which have been arrested in an early stage of B-cell differentiation. A key obstacle in identifying biomarkers of malignant B-cells in ALL patients has been constructing a cDNA library of normal B-lineage committed lymphoblasts from pediatric bone marrow for comparison. In order to identify unique biomarkers, a relatively quick method for isolating B cell subsets from pediatric bone marrow for RNA expression profiling is in development.