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dc.contributor.advisorYang, Bingeng
dc.contributor.authorTeme, Mariameng
dc.date.issued2022eng
dc.date.submitted2022 Falleng
dc.description.abstractCausative agent of black rot of crucifers, Xanthomonas campestris pv. campestris (Xcc), is a rod-shaped Gram-negative bacterium that belongs to the [gamma]-subdivision of Proteobacteria. It was first described in 1889 by the entomologist and botanist Harrison Garman in Kentucky, USA. Xcc is nowadays one of the most economically damaging bacterial pathogens to plants of the family Brassicaceae. In addition, some accessions of the model plant Arabidopsis thaliana respond differently when infected with this bacterium. In this study, we investigate different responses of Arabidopsis ecotypes to Xanthomonas campestris pv. campestris. Two resistant ecotypes Drahonin (Dra-0) and Wassilewskija (Ws-2)) were identified using colony forming unit assay. Bacterial pathogens depend on different strategies (e.g., secretion systems) to invade and colonize their hosts. The pathogenicity of Xcc is correlated with the type 3 secretion system (T3SS) which is a protein appendage used by bacteria to transfer effector proteins into the host cells for virulence. Xanthomonas T3SS effector genes are classified into different groups called Xanthomonas Outer Proteins (Xops). xopAC is a major avirulence gene of Xanthomonas campestris pv. campestris in Arabidopsis ecotype Columbia 0. The second part of the research aims to study the interaction between Xanthomonas campestris pv. campestris 2669[delta]xopAC and different Arabidopsis mutants. Bioluminescence-based quantitative detection of bacteria in plants and colony counting assay are performed and compared to determine which method gives more accuracy and is less time consuming. The software ImageJ used to quantify the bioluminescence images was not as precise as expected. The transposon-based bioluminescence for quantitative detection of bacterial titer in plants was also successfully applied to the bacterium Pseudomonas syringae pv. tomato.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentxii, 69 pages : illustrations (color)eng
dc.identifier.urihttps://hdl.handle.net/10355/95169
dc.identifier.urihttps://doi.org/10.32469/10355/95169eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.titleArabidopsis and Xanthomonas campestris pv. campestris two model organisms in plant pathologyeng
dc.typeThesiseng
thesis.degree.disciplinePlant Insect and Microbial Sciences (MU)eng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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