Development of a novel fluorescent sensor for detection of 4-hydroxynonenal

Abstract

[EMBARGOED UNTIL 12/1/2023] 4-Hydroxynonenal (4-HNE) is one of the secondary products of lipid peroxidation and has been studied in the greatest detail, due to its high reactivity and consequently, its role in a wide range of biological processes. During oxidative stress, reactive oxygen species involved in a free radical chain mechanism can induce the production of lipid peroxyl radicals, hydroperoxides and a few toxic secondary byproducts such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE). the latter byproduct is the target analyte for our proposed sensor. In this project, the goal is to design a turn on fluorescent sensor that could quantitate 4-HNE levels with high selectivity in serum. Toward this aim, we plan to develop a thiol-based sensor that enables us to take advantage of thiol- Michael addition reaction with our target analyte, 4-HNE. dansyl was selected as the fluorophore for this sensor.