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dc.contributor.advisorKelley, Cheryl A., 1961-eng
dc.contributor.authorPoole, Jennifer A., 1985-eng
dc.coverage.spatialCalifornia -- Elkhorn Slougheng
dc.coverage.spatialCalifornia -- Don Edwards San Francisco Bay National Wildlife Refugeeng
dc.coverage.spatialMexico -- Guerrero Negroeng
dc.date.issued2010eng
dc.date.submitted2010 Summereng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on August 12, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Cheryl Kelley.eng
dc.descriptionM.S. University of Missouri--Columbia 2010.eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The central goal of this study was to determine the dominant substrates used by methanogens in the salt ponds of Elkhorn Slough, the Don Edwards National Wildlife Refuge, and the and the Exportadora de Sal saltern. Microbial mat and/or sediments were amended with site water only or with site water and various substrates and incubated in N2(g) flushed serum vials. It was hypothesized that trimethylamine (TMA) would be a dominant substrate in all ponds. Additionally, samples were incubated with substrates also reported to be of importance in hypersaline environments: methanol (MeOH); dimethylsulfide (DMS); monomethylamine (MMA); and acetate. As expected, high concentrations of TMA and MMA stimulated methane production to a greater extent than the controls across the entire salinity gradient sampled and there was also demonstrated use of 13C-labeled methanol at all of the Guerrero Negro sites. Apparent fractionation factors were calculated to determine if they could be used to predict dominant substrates of hypersaline methanogenesis. Because TMA and MeOH were used, apparent fractionation factors were expected to be high and in the range of 1.04 to 1.09 (the published range for TMA and MeOH). However, calculated apparent fractionation factors were lower and ranged from 1.02 to 1.07, indicating use of other substrates. The difference in expected versus measured apparent fractionation factors could be a result of methanogens being substrate limited because of high salinities or low POC concentrations, thus reducing the expressed fractionation of the stable isotopes.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.format.extentxii, 88 pageseng
dc.identifier.merlinb81029524eng
dc.identifier.oclc694518489eng
dc.identifier.urihttps://hdl.handle.net/10355/9524
dc.identifier.urihttps://doi.org/10.32469/10355/9524eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campuses of the University of Missouri.eng
dc.subjectExportadora de Sal saltern, Guerrero, Negro, Baja California, Sur, Mexicoeng
dc.subject.lcshMethanoleng
dc.subject.lcshDimethyl sulfideeng
dc.subject.lcshBicarbonate ionseng
dc.subject.lcshAcetateseng
dc.subject.lcshMethanobacteriaceaeeng
dc.subject.lcshMethanobacteriaceaeeng
dc.titleDominant substrates used by methanogens in hypersaline environmentseng
dc.typeThesiseng
thesis.degree.disciplineGeological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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