Cell lines for the study of bone formation and regulation [abstract]
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UMKC Researchers have developed novel cell lines that are useful in the examination of osteocyte function, biomineralization, SOST/sclerostin, FGF23 and other mechanisms of osteoblast-to-osteocyte differentiation. The two cell lines were isolated from long bone of a mouse that was generated by crossing the Immortomouse[trademark] with a mouse where the DMP1 promoter drives expression of the GFP.One of the cell lines, IDG-SW3 (SW3), expresses all of the markers of osteocytes including Dmp1-GFP, Dmp1, E11/gp38, SOST/sclerostin, and FGF23. The second cell line, IDG-TI (T1), mainly expresses the characteristics of the matrix producing osteoblast such as high alkaline phosphatase, with delayed expression of Dmp1-GFP and E11/gp38, but no expression of SOST/sclerostin or FGF23. Both cells will produce new bone in vivo. Potential Areas of Applications: * To generate large numbers of osteocyte-like cells in order to produce sufficient quantities of osteocytes for study. * To generate large numbers of cells of a homogeneous stage of osteogenic differentiation. * To study osteocyte secretion of sclerostin, such as screening for sclerostin antagonists. * To investigate regulation of FGF23 expression in osteocytes and the role of osteocytes in regulation blood calcium/phosphate homeostasis. * To study the role of osteocytes as mechanosensory cells and their role in regulating bone response to mechanical stress. * To screen potential new therapies to induce bone formation. * To track cells responsible for bone formation in vivo. * To identify additional osteocyte-selective markers and receptors. Patent Status: Provisional filed Inventor(s): Bonewald, Lynda F.; Woo, Stacey M. Contact Info: James Brazeal - brazealj@umkc.edu (816) 235-5091