Teaching T cells to remember: the role of antigen presenting cells in the development of CD4+ T cell memory
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[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Recent studies in our lab suggest that the number of divisions a naive T cell undergoes at the time of initial stimulation with antigen (Ag) affects that cell's fate as it relates to memory development. From this, it was hypothesized that the specific type of antigen presenting cell (APC) involved in the initial encounter with Ag affects the decision to become memory. To test this hypothesis, we have developed an adoptive T cell transfer model allowing us to investigate the role of specific APCs in the development of immunologic memory. Ovalbumin (OVA)- specific TCR transgenic CD4+ T cells were stimulated in vitro with Ag by purified, distinct types of APCs. These T cells were then transferred into naive mice lacking CD4+ T cells and parked for 4 mo. At this point, their memory response was analyzed upon recall with Ag. Interestingly, only B cells and CD8[alpha]+ dendritic cells (DCs) induced cells capable of inducing a strong memory cytokine response. This induction of memory was dependent on PD-L2 expression by the APCs. These findings indicate that the type of APC involved in the naive T cell's initial encounter with Ag influences the memory response.
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