Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Missouri Technology Expo (MU)
    • Missouri Technology Expo 2010 (MU)
    • Abstracts (Missouri Technology Expo 2010)
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Missouri Technology Expo (MU)
    • Missouri Technology Expo 2010 (MU)
    • Abstracts (Missouri Technology Expo 2010)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Nanopore-facilitated single molecule detection of circulating microRNAs in cancer patients

    Gu, Li-Qun
    Wang, Yong, 1980-
    Wang, Michael X.
    Zheng, Dali
    View/Open
    [PDF] NanoporeFacilitatedSingle[abstract].pdf (51.26Kb)
    [PDF] NanoporeFacilitiatedSingle.pdf (3.370Mb)
    Date
    2010-10
    Format
    Presentation
    Metadata
    [+] Show full item record
    Abstract
    Developing new technologies for cancer screening and early diagnosis is a critical issue for saving cancer patients' lives. MicroRNAs (miRNAs) are a class of short (~18-24-nt) non-coding RNAs molecules that regulate gene expression at the post-transcriptional level. Aberrant expression of miRNAs has been found in all types of tumors. Thus miRNAs have been recognized as potential cancer biomarkers. Most notably, specific miRNAs are released from the primary tumor into blood circulation, making the detection of circulating miRNAs profile a powerful tool for noninvasive cancer detection, diagnosis, staging, and monitoring. We developed a robust nanopore sensor that selectively detects single molecules of circulating miRNAs derived from primary cancer. The nanopore is a fabricated 2-nm molecular pore. Such a tiny pore can generate a signature current signal when a miRNA molecule is specifically captured in it. These signals function as fingerprints that enable us to identify a specific miRNA and quantify its concentration. The prototype of nanopore sensor has demonstrated the capability to discriminate single nucleotide difference between miRNAs (single nucleotide polymorphisms, SNPs). In clinical tests, the nanopore has shown the power to differentiate miRNA levels in blood from lung cancer patients and healthy people. Due to the label-free single molecule detection without nucleic acids amplification, the nanopore sensor is higher selective, precise and accurate over the gold standard RT-PCR and microarray. This noninvasive clinical test requires a mere 5 ml of peripheral blood, with a reduced cost from several hundred dollars today to less than 20 dollars per sample. The developing nanopore array would give a high throughput capability for detecting miRNA profile. If validated in clinical trial, the nanopore sensor will become a system available to monitor cancer patients and to screen high risk populations for early diagnosis of cancers which will potentially save the lives of millions. Potential Areas of Applications: * Detection of microRNAs, study of DNA, RNA, DNA/RNA hybrid unzipping kinetics * Study of miRNA mechanism and principle of regulation * Drug discovery and development * Biomarker characterization * Diagnostics and prognostics.
    URI
    http://hdl.handle.net/10355/9764
    Part of
    Abstracts (Missouri Technology Expo 2010)
    Collections
    • Presentations (Missouri Technology Expo 2010)
    • Abstracts (Missouri Technology Expo 2010)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems