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dc.contributor.advisorCritser, John Kennethen_US
dc.contributor.authorBenson, Corinna Mary Kashuba, 1971-en_US
dc.date.issued2009eng
dc.date.submitted2009 Fallen_US
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on January 25, 2011).en_US
dc.descriptionIncludes bibliographical referencesen_US
dc.descriptionVita.en_US
dc.descriptionThesis advisor: John K. Critser.en_US
dc.description"December 2009"en_US
dc.descriptionPh. D. University of Missouri-Columbia 2009.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary pathobiology area program.en_US
dc.description.abstractMouse embryonic stem cell (mESC) lines are central to projects such as the Knock-Out Mouse Project, which seek to create thousands of mutant mouse strains using mESCs for the production of human disease models. The ability to efficiently cryopreserve these cell lines for banking and transport is crucial to the success of these programs. The post-thaw recovery of viable cells varies significantly by genetic background, therefore there is a need to improve the efficiency and reduce the variability of current mESC cryopreservation methods. We employed the principles of fundamental cryobiology to improve the cryopreservation protocol of five mESC lines from different genetic backgrounds (BALB/c, C57BL/6, CBA, FVB, and 129R1 mESCs). Using methods outlined in this dissertation, a protocol utilizing 1 M propylene glycol, a cooling rate of 1°C/minute, and plunge into liquid nitrogen at -41°C, combined with subsequent warming in a 22°C water bath significantly improved post-thaw recovery for most mESC lines. Additionally, the effects of Latrunculin A (LATA), 1.5 M dimethyl sulfoxide (Me2SO), and temperature were examined on C57BL/6 mESC osmotic response and permeability parameters. Temperature, Me2SO, and LATA significantly influenced isosmotic cell volume, and LATA significantly affected adjusted osmotically inactive cell volume as well as permeability parameters for the C57BL/6 mESC line.en_US
dc.format.extentxi, 159 pagesen_US
dc.identifier.oclc698474012en_US
dc.identifier.otherKashubaC-121409-D815en_US
dc.identifier.urihttp://hdl.handle.net/10355/9881
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartof2009 Freely available dissertations (MU)en_US
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2009 Dissertations
dc.subject.lcshCryopreservation of organs, tissues, etc. -- Methodsen_US
dc.subject.lcshCryobiology -- Methodsen_US
dc.subject.lcshEmbryonic stem cells -- Cryopreservationen_US
dc.subject.lcshTransgenic miceen_US
dc.subject.lcshGlycolsen_US
dc.subject.lcshThiazolesen_US
dc.subject.lcshDimethyl sulfoxideen_US
dc.titleComparative fundamental cryobiology of mouse embryonic stem cellsen_US
dc.typeThesisen_US
thesis.degree.disciplineVeterinary pathobiology area programen_US
thesis.degree.disciplineVeterinary pathobiology area programeng
thesis.degree.grantorUniversity of Missouri--Columbiaen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePh. D.en_US


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