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dc.contributor.advisorCritser, John Kennetheng
dc.contributor.authorBenson, Corinna Mary Kashuba, 1971-eng
dc.date.issued2009eng
dc.date.submitted2009 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on January 25, 2011).eng
dc.descriptionIncludes bibliographical referenceseng
dc.descriptionVita.eng
dc.descriptionThesis advisor: John K. Critser.eng
dc.description"December 2009"eng
dc.descriptionPh. D. University of Missouri-Columbia 2009.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary pathobiology area program.eng
dc.description.abstractMouse embryonic stem cell (mESC) lines are central to projects such as the Knock-Out Mouse Project, which seek to create thousands of mutant mouse strains using mESCs for the production of human disease models. The ability to efficiently cryopreserve these cell lines for banking and transport is crucial to the success of these programs. The post-thaw recovery of viable cells varies significantly by genetic background, therefore there is a need to improve the efficiency and reduce the variability of current mESC cryopreservation methods. We employed the principles of fundamental cryobiology to improve the cryopreservation protocol of five mESC lines from different genetic backgrounds (BALB/c, C57BL/6, CBA, FVB, and 129R1 mESCs). Using methods outlined in this dissertation, a protocol utilizing 1 M propylene glycol, a cooling rate of 1°C/minute, and plunge into liquid nitrogen at -41°C, combined with subsequent warming in a 22°C water bath significantly improved post-thaw recovery for most mESC lines. Additionally, the effects of Latrunculin A (LATA), 1.5 M dimethyl sulfoxide (Me2SO), and temperature were examined on C57BL/6 mESC osmotic response and permeability parameters. Temperature, Me2SO, and LATA significantly influenced isosmotic cell volume, and LATA significantly affected adjusted osmotically inactive cell volume as well as permeability parameters for the C57BL/6 mESC line.eng
dc.format.extentxi, 159 pageseng
dc.identifier.oclc698474012eng
dc.identifier.otherKashubaC-121409-D815eng
dc.identifier.urihttp://hdl.handle.net/10355/9881eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2009 Freely available dissertations (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2009 Dissertationseng
dc.subject.lcshCryopreservation of organs, tissues, etc. -- Methodseng
dc.subject.lcshCryobiology -- Methodseng
dc.subject.lcshEmbryonic stem cells -- Cryopreservationeng
dc.subject.lcshTransgenic miceeng
dc.subject.lcshGlycolseng
dc.subject.lcshThiazoleseng
dc.subject.lcshDimethyl sulfoxideeng
dc.titleComparative fundamental cryobiology of mouse embryonic stem cellseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary pathobiology area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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