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dc.contributor.authorBodkin, Nicole C.eng
dc.contributor.authorJia, Fangeng
dc.contributor.authorLewis, Michael R.eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia)eng
dc.date2007eng
dc.date.issued2007eng
dc.descriptionAbstract only availableeng
dc.description.abstractThe B-cell lymphoma/leukemia-2 (bcl-2) gene is overexpressed in non-Hodgkin's lymphoma. It produces a protein that blocks apoptosis and is strongly correlated with increased relapse rates after chemotherapy and poor overall survival. In this study, radiolabeled peptide nucleic acid (PNA) probes were delivered to bcl-2 mRNA-deficient and -rich tumor models in mice to determine whether the probes are specific for cells that express bcl-2 mRNA and to differentiate between positive and negative bcl-2 tumors. A peptide, Tyr3-octreotate, was attached to the PNA to target somatostatin receptors on the surface of tumor cells, where the PNA can then be delivered into the cell and bind to bcl-2 mRNA. In vitro efflux studies of 111In-DOTA-anti-bcl-2-PNA-Tyr3-octreotate were performed in somatostatin receptor positive Mec-1 (bcl-2 positive) and Ramos (bcl-2 negative) cells. The cell associated radioactivity was 14.9% in Ramos cells, as compared with 60% in Mec-1 cells at 4 h post-injection, signifying that PNA is washed out of the Ramos cells while there is higher retention in Mec-1 cells. In in vivo studies, 111In-DOTA-anti-bcl-2-PNA-Tyr3-ocreotate and a modified compound, 64Cu-DOTA-anti-bcl-2-PNA-T(s)-Tyr3-octreotate, were injected into Ramos-bearing nude mice and Mec-1-bearing SCID mice, and organ uptakes were compared at 48 h post-injection. The results showed a 0.18% injected dose per gram of tissue (ID/g) tumor uptake with the 111In labeled compound in Ramos mice and 1.33% ID/g in Mec-1 mice. The 64Cu labeled compound in the Ramos mice showed a 0.69% ID/g tumor uptake and 1.12% ID/g in Mec-1 mice. Both studies showed a significantly lower tumor uptake in Ramos mice than in Mec-1 mice, concluding that the PNA in both compounds are specific for bcl-2 mRNA.eng
dc.identifier.urihttp://hdl.handle.net/10355/988eng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectlymphomaeng
dc.titleIn vivo and in vitro analysis of In-111 and Cu-64 labeled PNA-peptides in mice [abstract]eng
dc.typePresentationeng


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