Dalton Cardiovascular Research Center presentations and publications (MU)
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Item Sedentary death syndrome is what researchers now call America's second largest threat to public health(2001) Booth, Frank W., Ph. D.; Krupa, Donna J.Obesity has doubled, Type 2 diabetes has increased nine-fold, and heart disease remains the number one cause of death for Americans. Sedentary Death Syndrome, or "SeDS," is a growing list of health disorders that are exacerbated by lack of physical activity, causing premature disability and death. Sixty percent of all Americans are at risk, including children. SeDS is expected to add as much as $3 trillion to healthcare costs over ten years, more than twice the tax cut passed by the US Senate.Item Cost and Consequences of Sedentary Living: New Battleground for an Old Enemy(2002) Booth, Frank W., Ph. D.; Chakravarthy, Manu V.The purpose of this review is to update our earlier review by itemizing, as best we can, the costs and consequences of sedentary living, and thus provide cost reasons to fight a war against sedentary lifestyles.Item Integration of exercise sciences research: Achieving a balance between reductionist versus integrative approaches(2000) Booth, Frank W., Ph. D.This presentation argues that exercise sciences will badly miss achieving a balance between reductionistic versus integrative approaches under the current NIH plan to reorganize NIH study sections. NIH wants to score exercise grants in multiple study sections whose mentality does not appreciate the balance between reductionism and integration in exercise to score exercise grants against grants better appreciated by the group dynamics of that study section.Item Novel Nanostructured Organosilicate Nanoparticle Coatings for Chem-Bio Sensing [abstract](2010-03) Korampally, Venumadhav, 1972-; Darr, Charles Matthew, 1984-; Polo-Parada, Luis; Gangopadhyay, Keshab; Gangopadhyay, Shubhra; Grant, Sheila Ann; Sobel, Annette; Singh, Balram; University of Missouri (System); Missouri Life Sciences Summit (2010: University of Missouri--Kansas City)We present novel nanostructured organosilicate particulate based films and demonstrate that these materials have a great potential for chemical-biological sensor development. With unprecedented high surface areas (> 1400 m2/g) and optical transparency together with its easy surface functionalization, these materials can be readily interfaced with existing immunoassays for the rapid and trace detection of both chemical and biological warfare agents. The ultra high surface area associated with these films stems from its unique nanostructure consisting of nanoparticles (2-5nm) in a “raspberry” structure in combination with interconnected nanopores (3-10nm). This unique nanostructure has been exploited to immobilize high areal density of sensor probes to improve the sensing performance. Two orders of magnitude increase in binding density was achieved when fluorescently tagged protein A molecules were immobilized upon these surfaces compared to flat substrates (glass and Silicon). Our on-going work applies these materials to develop platforms for multiplexed sensitive detection of biological and chemical agents at point of care for both army and civilian use.Item Shock Wave Based Cell Transfection and Fluorescent Organosilicate Nanoparticles for Targeted Drug Delivery [abstract](2010) Korampally, Madhuri; Apperson, Steven J., 1982-; Korampally, Venumadhav, 1972-; Bok, Sangho, 1972-; Thiruvengadathan, Rajagopalan; Bezmelnitsyn, Andrey; Polo-Parada, Luis; Gangopadhyay, Keshab; Gangopadhyay, Shubhra; University of Missouri (System); Missouri Life Sciences Summit (2010: University of Missouri--Kansas City)Nanotechnology is a multidisciplinary field that has applications in life sciences, alternative energy, national defense, and electronics. In the field of medicine, nanotechnology may enable intelligent drug delivery using multifunctional nanoparticles. Here, we show two technologies that are envisioned to work in tandem to enable targeted detection and treatment. First, a shock wave generator used for cell transfection and drug/particle delivery is presented. Then, fluorescent dye/drug encapsulated organosilicate nanoparticles (OSNP) with functionalized surfaces for targeted delivery are described. The shock wave generator has been successfully used to deliver various molecules and nanoparticle to inside of the cells with very high efficiency and low cell damage. These include dextran (77 kDa), naked plasmid, and dye-doped organosilicate nanoparticles into several types of cells lines including T47-D, HL-60, and MCF-7, and also into tissues including entire chicken heart (at developmental stage 20-30) and chicken spinal cord. Dye doped organosilicate nanoparticle surfaces conjugated to antibodies have been successfully used in immunofluorescence assays. Close examination of the nanostructure of these particles reveal its unique nanoporous structure. These nanoparticles are currently under investigation for drug encapsulation and sustained release. The implication of these technologies is that the OSNP can be used as targeted drug carriers, and the shock wave generator can be used to deliver the OSNP into cells to which the particles attach. The research on shock wave micro-transfector system has been funded by the National Science Foundation Grant Opportunities for Academic Liason with Industries program.