Chemistry Department publications, presentations, and datasets (MU)
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Items in this collection represent publications, public presentations, and research products from the Department of Chemistry faculty, staff, and students, either alone or as co-authors. The items may have been published in an alternate format and may contain more than one file type.
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Item Quercetin potentiates docosahexaenoic acid to suppress lipopolysaccharide-induced oxidative/inflammatory responses, alter lipid peroxidation products, and enhance the adaptive stress pathways in BV-2 microglial cells(MDPI AG, 2019) Sun, G.Y.; Li, R.; Yang, B.; Fritsche, K.L.; Beversdorf, D.Q.; Lubahn, D.B.; Geng, X.; Lee, J.C.; Michael, Greenlief C.; ChemistryHigh levels of docosahexaenoic acid (DHA) in the phospholipids of mammalian brain have generated increasing interest in the search for its role in regulating brain functions. Recent studies have provided evidence for enhanced protective effects when DHA is administered in combination with phytochemicals, such as quercetin. DHA and quercetin can individually suppress lipopolysaccharide (LPS)-induced oxidative/inflammatory responses and enhance the antioxidative stress pathway involving nuclear factor erythroid-2 related factor 2 (Nrf2). However, studies with BV-2 microglial cells indicated rather high concentrations of DHA (IC 50 in the range of 60-80 µM) were needed to produce protective effects. To determine whether quercetin combined with DHA can lower the levels of DHA needed to produce protective effects in these cells is the goal for this study. Results showed that low concentrations of quercetin (2.5 µM), in combination with DHA (10 µM), could more effectively enhance the expression of Nrf2 and heme oxygenase 1 (HO-1), and suppress LPS-induced nitric oxide, tumor necrosis factor-[alpha], phospho-cytosolic phospholipase A 2 , reactive oxygen species, and 4-hydroxynonenal, as compared to the same levels of DHA or quercetin alone. These results provide evidence for the beneficial effects of quercetin in combination with DHA, and further suggest their potential as nutraceuticals for improving health.Item Maternal dietary docosahexaenoic acid alters lipid peroxidation products and (N-3)/(n-6) fatty acid balance in offspring mice(MDPI AG, 2019) Yang, B.; Li, R.; Woo, T.; Browning, J. D.; Jr.; Song, H.; Gu, Z.; Cui, J.; Lee, J. C.; Fritsche, K. L.; Beversdorf, D. Q.; Sun, G. Y.; Greenlief, C. M.; ChemistryThe abundance of docosahexaenoic acid (DHA) in the mammalian brain has generated substantial interest in the search for its roles in regulating brain functions. Our recent study with a gene/stress mouse model provided evidence to support the ability for the maternal supplement of DHA to alleviate autism-associated behavior in the offspring. DHA and arachidonic acid (ARA) are substrates of enzymatic and non-enzymatic reactions, and lipid peroxidation results in the production of 4-hydroxyhexenal (4-HHE) and 4-hydroxynonenal (4-HNE), respectively. In this study, we examine whether a maternal DHA-supplemented diet alters fatty acids (FAs), as well as lipid peroxidation products in the pup brain, heart and plasma by a targeted metabolite approach. Pups in the maternal DHA-supplemented diet group showed an increase in DHA and a concomitant decrease in ARA in all brain regions examined. However, significant increases in 4-HHE, and not 4-HNE, were found mainly in the cerebral cortex and hippocampus. Analysis of heart and plasma showed large increases in DHA and 4-HHE, but a significant decrease in 4-HNE levels only in plasma. Taken together, the DHA-supplemented maternal diet alters the (n-3)/(n-6) FA ratio, and increases 4-HHE levels in pup brain, heart and plasma. These effects may contribute to the beneficial effects of DHA on neurodevelopment, as well as functional changes in other body organs.Item Comparative proteomic analysis unveils critical pathways underlying the role of Nitrogen fertilizer treatment in American elderberry(MDPI AG, 2019) Yang, B.; Thomas, A. L.; Greenlief, C. M.; ChemistryAmerican elderberry (Sambucus nigra subsp. canadensis) is a rapidly growing specialty crop in Missouri and eastern North America. Nitrogen (N) is a major nutrient involved in plant growth and development. However, proteome changes for different genotypes of elder in response to varying levels of N-treatment remain undefined. To reveal plant responses to N, comparative proteomic analyses were performed to determine consistent changes in three genotypes of elderberry leaves (Adams II, Bob Gordon and Wyldewood) grown under different N-fertilizer treatments. 165 proteins separated by two dimensional gel electrophoresis showed significant differences in abundance (p < 0.05 and greater than 2-fold). Principal component analysis of the abundance profiles of these proteins revealed Bob Gordon as a distinct genotype. The 165 proteins were identified by mass spectrometry and showed similar functional distributions in these genotypes underlying the N-treatment. Among the proteins identified, 23 are mainly involved in photosynthesis, protein metabolism and redox homeostasis. Their abundance profiles were not altered upon exposure to N or genotype. These results provide novel insights into plant responses to fertilizer treatment at the proteome level and could lead to a better understanding of molecular mechanisms of elderberry growth.Item Synthesis and Reactivity of 1,2-Bis(di-iso-propylphosphino)benzene Nickel Complexes: A Study of Catalytic CO2-Ethylene Coupling [Datasets](2018) Hopkins, M. N.; University of Missouri-Columbia. College of Arts and Sciences. Department of Chemistry.Single crystal X-ray diffraction photographs and data reduction output files in proprietary format, collected on a Bruker D8 Venture diffractometer with a Photon 100 detector using the Apex3 software suite.Item Formation of water channels in the crystalline hydrates of macrocyclic compounds [dataset](2018) Patil, Rahul S.; Zhang, Chen; Sikligar, Kanishka; Baker, Gary A.; Atwood, Jerry L.; University of Missouri-Columbia. College of Arts and Sciences. Department of Chemistry.RSP and matrix zip files contain .sfrm files that have both the photographic data and metadata desicribing the instrument, readable with scientific software. The work zip file subfolder contains a .p4p file generated after data reduction.