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Abundance of TRIM28, SETDB1, and TP53 mRNA is dynamically regulated during porcine early embryogenesis and is abnormal in preimplantation embryos produced by in vitro fertilization in comparison to in vivo derived and nuclear transfer derived embryos
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In vitro embryo production is important for research in animal reproduction, embryo transfer, transgenics, and cloning. However, in vitro-derived embryos ...
Transcriptional profiling to improve porcine preimplantation embryo development in vitro
(University of Missouri--Columbia, 2014)
Culture environments can have profound impacts on the developmental program of the preimplantation embryo. Even though significant improvements have been made to embryo culture environments they are still sub-optimal to ...
Metabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfer
(University of Missouri--Columbia, 2017)
Gene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. ...
Improved cloning efficiency by chemically induced metabolic reprogramming of donor cells used for porcine somatic cell nuclear transfer
(University of Missouri--Columbia, 2019)
The use of somatic cell nuclear transfer (SCNT) to create agricultural and biomedical models is extremely useful for understanding how we can improve the efficiency of production agriculture, as well as how we can better ...
Stem cells and DNA methylation reprogramming in pigs
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In mammals, the majority of cells in the body contain the same genome as other cells. The concept of nuclear totipotency has been well demonstrated by ...
The role of cytoplasmic polyadenylation element sequence on mRNA abundance in porcine embryogenesis
(University of Missouri--Columbia, 2010)
Development of a porcine germinal vesicle oocyte (GVO) to a 4-cell stage embryo occurs during a transcriptionally silent period when the oocyte/embryo relies on maternally derived mRNA to encode proteins required for ...
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...