Search
Now showing items 1-9 of 9
Mechanisms of clostridial toxin binding and translocation
(University of Missouri--Columbia, 2018)
The bacterial genus Clostridium consists of over 150 species of anaerobic, fermentative, spore-forming bacilli. Clostridial species produce up to 20% of all known bacterial exotoxins, which serve as important virulence ...
Structural inferences for reverse transcribing viruses and drug resistance
(University of Missouri--Columbia, 2019)
function. Chapter 4 will consolidate the protocols developed to solubilize the RTBV RT and build a firm understanding of the viral life cycle through determination of its characteristic enzyme functions with emphasis on divalent metal ion mediated catalysis...
Labeling HIV-1 nucleic acids during infection and effect of MOV10 in HBV replication
(University of Missouri--Columbia, 2018)
Technical limitations in simultaneous microscopic visualization of RNA, DNA, and proteins of HIV have curtailed progress in this field. To address this need we have developed a microscopy approach, multiplex immunofluorescent ...
Protein processing strategies by adeno-associated virus type 5 (AAV5) and the effects of the adenovirus E4orf6/E1b-55k/Cullin 5 E3 ubiquitin ligase complex on AAV protein stability
(University of Missouri--Columbia, 2008)
We report the initial identification of the ubiquitination of a parvovirus non-structural protein by the adenovirus E4orf6/E1b-55k/Cullin 5 E3 ubiquitin ligase complex. The small Rep and capsid proteins of adeno-associated virus type 5 (AAV5) were...
Comparative study of Human Immunodeficiency Virus type 1 and type 2 single and dual infections
(University of Missouri--Columbia, 2020)
active chromatin marker H3K9me2 levels, and should be equally accessible to host transcription machinery. Host RNA polymerase II (RNAP II) occupancy analysis at each LTR promoter start sites showed less occupancy in the case of HIV-2 LTR. Secondly, we...
Duchenne muscular dystrophy gene therapy in the canine model
(University of Missouri--Columbia, 2018)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Duchenne muscular dystrophy (DMD) is a devastating muscle wasting disease caused by loss of function mutations in the dystrophin gene, resulting in the ...
Refinement of an established large-animal model to understand the tick-pathogen-host interface
(University of Missouri--Columbia, 2021)
with tick salivary gland tissues. The proteins identified here are important for future experiments to determine their posited roles in reduction of tick feeding. Finally, the research in chapter 4 built on this work and suggested that immunization...
Reparative dysfunction during muscle regeneration in Duchenne muscular dystrophy: therapeutic approaches to modulating the muscle environment and muscle stem cells
(University of Missouri--Columbia, 2019)
the environment by improving fibrosis, stiffness, and fiber orientation, which significantly improved early muscle regeneration but not late regeneration (2 [greater than] and [less than] 4 months post-transplant) via enhancing muscle stem cells differentiation...
Establishing the posh scaffold as a novel therapeutic target for treatment of B cell leukemia
(University of Missouri--Columbia, 2020)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT REQUEST OF AUTHOR.] More than 60,000 new cases of leukemia will be diagnosed in the U.S. this year. B cells comprise 10 [percent] of acute lymphoblastic leukemias ...