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Increasing methionine content of soybean using CRISPR/Cas9 and developing machine learning predictive models
(University of Missouri--Columbia, 2022)
that the dual-gRNA CRISPR/Cas9 system indeed offers a rapid and highly efficient genetic tool to knockout multiple genes simultaneously. Knock out mutations in three GmMGLs genes (GmMGL1, GmMGL2 and GmMGL3) were simultaneously created and, as predicted...