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Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2-1A and GmFAD2-1B genes to yield a high oleic, low linoleic and ?-linolenic acid phenotype in soybean
(BioMed Central Ltd., 2019)
) that converts the monounsaturated oleic acid (C18:1) to the polyunsaturated linoleic acid (C18:2), therefore, regulating the content of monounsaturated fats in soybean seeds. Results: We designed two gRNAs to guide Cas9 to simultaneously cleave two sites, spaced...