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Simultaneous quantitation of Escherichia coli O157:H7, salmonella and shigella in ground beef by multiplex real-time PCR and immunomagnetic separation
(University of Missouri--Columbia, 2006)
outbreaks. Genomic DNA for the real-time PCR was extracted by the boiling method. Three sets of primers and corresponding TaqMan® probes were designed to target these three pathogens. Multiplex real-time PCR was carried out with TaqMan® Universal PCR Master...