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Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
Glutamine metabolism and interactions with mechanistic target of rapamycin complex 1 in porcine preimplantation embryos
(University of Missouri--Columbia, 2021)
The ability to culture embryos has created opportunities for animal agriculture, including the refined selection of superior genetics, and for the medical field, allowing couples experiencing infertility to have biological ...
Comparative transcriptional profiling of the uterus according to stage of the estrous cycle and pregnancy status in gilts
(University of Missouri--Columbia, 2007)
In most mammals, female reproductive tracts undergo extensive tissue remodeling throughout the estrous cycle and during pregnancy that arise in response to an array of endocrine, paracrine, and autocrine signals. These ...
Identification and quantification of differentally represented transcripts in preimplantation bovine embryos
(University of Missouri--Columbia, 2007)
Identification of transcripts present at key development stages in preimplantation embryos is critical for a better understanding of early embryogenesis. To that end, we characterized the relative abundance of multiple ...
Improving viability of immunodeficient RAG2-/- pigs by non-specific porcine antibody administration
(University of Missouri--Columbia, 2020)
was reported in our immunocompetent wildtype pigs as remaining present in vivo over 0.05 [mu]g/mL for 10 days yielding a half-life of approximately 3.5 days, even though IgG has been described to have a half-life of 21 days....
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...
The effects of elevated temperature on preimplantation-stage porcine embryos
(University of Missouri--Columbia, 2006)
It was the objective of these experiments to more fully characterize the biochemical and developmental responses of in vitro-produced preimplantation stage porcine embryos to elevated temperatures. In vitro fertilized (IVF) ...
DNA methylation in the early porcine embryo
(University of Missouri--Columbia, 2007)
Reproductive technologies such as in vitro fertilization, intracytoplasmic sperm injection, parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, ...