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Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos
(University of Missouri--Columbia, 2006)
Nuclear transfer (NT) is an inefficient but invaluable tool. This study examines abnormalities associated with NT porcine embryos at days 10, 12, and 14. Methods: 4 experimental groups were examined: non-pregnant, in vivo ...
Metabolic programming of a Warburg effect-like phenotype in donor fibroblasts prior to somatic cell nuclear transfer
(University of Missouri--Columbia, 2017)
Gene edited pigs serve as excellent models for biomedicine and agriculture. Currently, the most efficient way to make a reliably-edited transgenic animal is through somatic cell nuclear transfer (SCNT) also known as cloning. ...
Stem cells and DNA methylation reprogramming in pigs
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In mammals, the majority of cells in the body contain the same genome as other cells. The concept of nuclear totipotency has been well demonstrated by ...
The role of cytoplasmic polyadenylation element sequence on mRNA abundance in porcine embryogenesis
(University of Missouri--Columbia, 2010)
Development of a porcine germinal vesicle oocyte (GVO) to a 4-cell stage embryo occurs during a transcriptionally silent period when the oocyte/embryo relies on maternally derived mRNA to encode proteins required for ...
Transcriptional profiling to improve porcine preimplantation embryo development in vitro
(University of Missouri--Columbia, 2014)
Culture environments can have profound impacts on the developmental program of the preimplantation embryo. Even though significant improvements have been made to embryo culture environments they are still sub-optimal to ...
Comparative transcriptional profiling of the uterus according to stage of the estrous cycle and pregnancy status in gilts
(University of Missouri--Columbia, 2007)
In most mammals, female reproductive tracts undergo extensive tissue remodeling throughout the estrous cycle and during pregnancy that arise in response to an array of endocrine, paracrine, and autocrine signals. These ...
Identification and quantification of differentally represented transcripts in preimplantation bovine embryos
(University of Missouri--Columbia, 2007)
Identification of transcripts present at key development stages in preimplantation embryos is critical for a better understanding of early embryogenesis. To that end, we characterized the relative abundance of multiple ...
Improving viability of immunodeficient RAG2-/- pigs by non-specific porcine antibody administration
(University of Missouri--Columbia, 2020)
was reported in our immunocompetent wildtype pigs as remaining present in vivo over 0.05 [mu]g/mL for 10 days yielding a half-life of approximately 3.5 days, even though IgG has been described to have a half-life of 21 days....
The effects of elevated temperature on preimplantation-stage porcine embryos
(University of Missouri--Columbia, 2006)
It was the objective of these experiments to more fully characterize the biochemical and developmental responses of in vitro-produced preimplantation stage porcine embryos to elevated temperatures. In vitro fertilized (IVF) ...
Abundance of TRIM28, SETDB1, and TP53 mRNA is dynamically regulated during porcine early embryogenesis and is abnormal in preimplantation embryos produced by in vitro fertilization in comparison to in vivo derived and nuclear transfer derived embryos
(University of Missouri--Columbia, 2013)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] In vitro embryo production is important for research in animal reproduction, embryo transfer, transgenics, and cloning. However, in vitro-derived embryos ...
Differential gene and protein expression in pig nuclear transfer extraembryonic membranes and blastocyst stage embryos
(University of Missouri--Columbia, 2010)
Nuclear transfer (cloning) is the process of transferring a donor cell nucleus into an enucleated oocyte. The oocyte and donor cell are subsequently fused and activated to allow for nuclear reprogramming and continued ...
Transcriptional profiling by deep sequencing indentifies [sic] differences in mRNA transcript abundance in in vivo derived vs. in vitro cultured porcine blastocyst stage embryos
(University of Missouri--Columbia, 2010)
In vitro embryo culture systems promote development at rates lower than in vivo. The goal of this project was to discover transcripts that may be responsible for a decrease of embryo competency in blastocyst stage embryos ...
DNA methylation in the early porcine embryo
(University of Missouri--Columbia, 2007)
Reproductive technologies such as in vitro fertilization, intracytoplasmic sperm injection, parthenogenetic activation, and somatic cell nuclear transfer are powerful procedures in the production of animals for agriculture, ...