Search
Now showing items 1-20 of 23
S-RNase proteins: functional studies of the 120kDa glycoprotein and SRNase oligomerization
(University of Missouri--Columbia, 2005)
Flowering plants control fertilization through pollen-pistil interactions. Self-incompatibility (SI) is a well-studied pollen-pistil interaction that promotes cross-pollination. SI is controlled by a multi-haplotype locus ...
Structural and functional studies of proline catabolic enzymes
(University of Missouri--Columbia, 2007)
Catabolism is the oxidation of organic nutrients into simple end molecules to extract energy. For proline, the catabolic pathway involves two enzymes, Lproline dehydrogenase (PRODH, EC 1.5.99.8) and L-[delta]¹-pyrroline- ...
Kinase-interacting FHA domain of kinase associated protein phosphatase : phosphopeptide interactions and NMR-detected dynamics
(University of Missouri--Columbia, 2007)
FHA domains are phosphoThr recognition modules found in diverse signaling proteins. Kinase-associated protein phosphatase (KAPP) from Arabidopsis employs its FHA domain in its negative regulation of some receptor-like ...
The role of P2Y₂ nucleotide receptors in vascular inflammation
(University of Missouri--Columbia, 2007)
Extracellular nucleotides act on P2 receptors to regulate vascular tone. Large amounts of extracellular nucleotides are released at the sites of tissue injury and may play an important role in the vascular inflammation. ...
Structure of an antigen-binding fragment bound to stem-loop DNA and crystallization of recombinant haemophilus influenzae e(P4) acid phosphatase
(University of Missouri--Columbia, 2006)
DNA-1 and 11F8 are anti-ss DNA antibodies derived from autoimmune lupus-prone mice. They are very similar to each other in terms of CDR sequence and preference for binding T-rich ss DNA. G1-17 is an oligonucleotide identified ...
Characterization and functional analysis of the P2Y₂R gene promoter
(University of Missouri--Columbia, 2006)
Extracellular nucleotides can bind to the P2Y₂R and modulate proliferation and migration of smooth muscle cells, which is known to be involved in intimal hyperplasia that accompanies atherosclerosis and post-angioplasty ...
Regulation of Nrf2 by a keap1-dependent E3 ubiquitin ligase
(University of Missouri--Columbia, 2007)
Oxidative damage to biological macromolecules has been implicated in diverse pathophysiological processes, including cancer, neurodegeneration, aging and cardiovascular disease. The Nrf2 transcription factor regulates ...
Protein phosphorylation regulation in Arabidopsis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Protein phosphorylation and dephosphorylation are fundamental mechanisms involved in many cell-signaling events. Coordinated actions of protein kinases ...
In vitro characterization of Nudt16 orthologs by sequence and function provide evidence supporting a nuclear RNA degradation pathway
(University of Missouri--Columbia, 2009)
Nudt16, previously called X29, is known to be a metal-dependent decapping enzyme expressed in mammals and Xenopus . Nudt16 has been proposed to be the catalytic component of a previously unknown nuclear RNA degradation ...
Characterization of SF-hERR[beta] repression of human estrogen receptor alpha and estrogen receptor-beta transcriptional activities
(University of Missouri--Columbia, 2009)
Discovery of new ways to modulate estrogen actions through estrogen receptor is always exciting because of the importance of estrogen and ERs in many human diseases. In this dissertation, we report our discovery of the ...
Structural studies of glyceraldehyde-3-phosphate dehydrogenase complexes and the E. coli PutA DNA binding domain
(University of Missouri--Columbia, 2006)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that catalyzes the formation of 1,3-bisphosphoglycerate from glyceraldehyde-3-phosphate. We have solved a high-resolution (1.75 Å) structure of a ...
Understanding Arabidopsis ion homeostasis in the post-genomic era : assigning function to two proteins involved in iron metabolism
(University of Missouri--Columbia, 2006)
Two projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 ...
Examination of metabolic and regulatory networks of desulfovibrio species
(University of Missouri--Columbia, 2004)
The sulfate-reducing bacteria are a morphologically diverse group of organisms characterized by the ability to couple the enzymatic reduction of sulfate to energy production and growth. This metabolic activity has profound ...
Characterization and functional significance of Msc2p & Zrg17p, which form a zinc transport complex in the endoplasmic reticulum of Saccharomyces cerevisiae
(University of Missouri--Columbia, 2004)
We demonstrate a novel interaction between two members of the cation diffusion facilitator (CDF) family in Saccharomyces cerevisiae: Msc2p and a newly recognized CDF family member, Zrg17p. Both Msc2p and Zrg17p have been ...
Identification of novel breast carcinoma and melanoma avid peptides for imaging
(University of Missouri--Columbia, 2009)
During the last decade, peptides and their derivatives have been employed as effective cancer imaging agents. We aimed to identify new peptides probes specific for melanoma and breast carcinoma by phage display technology. ...
Structural studies of PMM/PGM from Pseudomonas aeruginosa
(University of Missouri--Columbia, 2005)
The human pathogen Pseudomonas aeruginosa expresses a variety of cell surface polysaccharides, including alginate, lipopolysaccharide (LPS), and rhamnolipid. P. aeruginosa is the primary cause of chronic lung infections ...
Physiologic and metabolic interactions in the soybean/bradyrhizobium japonicum symbiosis
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The agronomically vital, nitrogen-fixing symbioses between soybean and the soil bacterium Bradyrhizobium japonicum is a model system for the study of ...
Molecular and cell biological studies of mammalian zinc transporters
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Zinc is an essential trace element in all organisms. The importance of the hZIP4 human zinc transporter is highlighted by its causative role in a ...
Signals affecting the urease status of plant-associated bacteria, Methylobacterium spp
(University of Missouri--Columbia, 2007)
Pink-Pigmented Facultative Methylotrophic bacteria (PPFMs, Methylobacterium spp.) have been found to be the most abundant microorganisms among phylloplane microflora, and have been recovered from all plants examined. I ...
A study of SecA : the motor of the bacterial secretion system by site-directed spin labeling and EPR
(University of Missouri--Columbia, 2008)
For more than 3 decades, the biochemical details of the Sec system of protein export have been teased out of Escherichia coli. Through these efforts we know that SecA, the ATPase of the Sec system, interacts with several ...