dc.contributor.advisor | Kleiboeker, Steven B. | eng |
dc.contributor.author | Garcia-Tapia, David, 1971- | eng |
dc.date.issued | 2006 | eng |
dc.date.submitted | 2006 Spring | eng |
dc.description | Title from title screen of research.pdf file (viewed on December 22, 2006) | eng |
dc.description | The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. | eng |
dc.description | "May 2006" | eng |
dc.description | Vita. | eng |
dc.description | Thesis (Ph. D.) University of Missouri-Columbia 2006. | eng |
dc.description.abstract | Since the introduction of West Nile virus into the United States in 1999, the virus has spread throughout the continental territory of the United States and it is suspected that the virus has moved present into Mexico and Canada. The emergence of this flavivirus in North America has resulted in an intense interest in the virus, leading to research efforts focused on the pathogenesis and possible treatments or methods for prevention of the disease. In the present studies, we demonstrate the ability of WNV to productively infect horse monocytes, CD4+ T lymphocytes as well as monocyte-derived macrophages. Along with these findings we report the ability of immune horse serum to induce antibody dependent enhancement of WNV infection of horse macrophages and mouse macrophages in vitro. The question of ADE in vivo was also addressed and we found that the sub-neutralizing dilutions of anti-WNV immune horse serum that induce ADE in vitro fail to induce the same effect in vivo. The serum induces protection, which is perhaps driven by the up-regulation of IL-12 in spleen during the earliest phase of infection. In brain the chemokines C-10 and MCP-5 are secreted earlier than other chemokines and cytokines, suggesting that those chemokines play an important role in the beginning of the encephalitis caused by WNV. High titers of virus in blood and spleen, as well as the ability of the virus to infect horse monocytes and CD4+ lymphocytes suggest that this virus spreads from subcutaneous tissue to the brain by a hematogenous route. | eng |
dc.description.bibref | Includes bibliographical references. | eng |
dc.identifier.merlin | b57475222 | eng |
dc.identifier.oclc | 77524183 | eng |
dc.identifier.uri | https://doi.org/10.32469/10355/4475 | eng |
dc.identifier.uri | https://hdl.handle.net/10355/4475 | |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | OpenAccess. | eng |
dc.subject.lcsh | West Nile virus | eng |
dc.subject.lcsh | Macrophages | eng |
dc.subject.lcsh | Viral antibodies | eng |
dc.title | The role of macrophages and anti-viral antibodies in West Nile virus pathogenesis | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Veterinary pathobiology (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Doctoral | eng |
thesis.degree.name | Ph. D. | eng |