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dc.contributor.advisorHu, Zhiqiang, 1966-eng
dc.contributor.authorYou, Jia, 1986-eng
dc.date.issued2010eng
dc.date.submitted2010 Springeng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 21, 2010).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Zhiqiang Hu.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.S. University of Missouri--Columbia 2010.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Civil engineering.eng
dc.description.abstractThe inhibitory effort of two commonly used nanoparticles, silver nanoparticles (Ag NPs) and zinc oxide nanoparticles (ZnO NPs), on the growth of bacteria (E. coli) and bacteriophage (MS2) were evaluated using a turbidimetric microtiter assay and the standard double agar layer (DAL) assay. In the pure E. coli cultures, Ag NPs presented a greater degree of inhibition against bacteria than ZnO NPs. However, both nanoparticles did not deactivate MS2 at the highest nanoparticle concentrations tested (5 mg/L total Ag and 20 mg/L ZnO). Instead, exposure of MS2 to ZnO NPs at the concentration of 20 mg/L ZnO resulted in significantly higher plaque forming units (PFU) than the control. No bacteriophage inactivation was observed in the presence of nanosilver, nanosilver/Ag+ mixture (50:50 of Ag+ and nanosilver in mass ratios) or Ag+ ions, all at the total Ag concentration of 5 mg/L. In a binary system containing bacteria and phages, both MS2 and Ag NPs reduced bacterial growth, but the degree of bacterial growth inhibition by nanosilver or a mixture of nanosilver/Ag+ was phage concentration dependent. For Ag+ ions at concentration of 5 mg/L Ag, complete bacterial growth inhibition was observed regardless of phage concentration. Results from the dynamic bacterial growth inferred from the turbidimetric microtiter assay and the parallel active bacterial and phage concentration measurements inferred from standard agar plate assay indicated that both Ag NPs and ZnO NPs facilitated MS2 to infect the E. coli host. The complex interactions among bacteria, phage and nanoparticles suggested that bacterial cell membrane disruption or structure change due to nanoparticle exposure might allow bacteriophage MS2 to enter bacterial host cells more easily and promote bacterial cell lysis.eng
dc.format.extentviii, 57 pageseng
dc.identifier.merlinb79483884eng
dc.identifier.oclc649821023eng
dc.identifier.urihttp://hdl.handle.net/10355/8073
dc.identifier.urihttps://doi.org/10.32469/10355/8073
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2010 Freely available theses (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2010 Theseseng
dc.subject.lcshNanoparticles -- Toxicologyeng
dc.subject.lcshSilvereng
dc.subject.lcshZinc oxideeng
dc.subject.lcshEscherichia colieng
dc.subject.lcshBacteriophageseng
dc.titleMetallic nanotoxicity to bacteria and bacteriophageseng
dc.typeThesiseng
thesis.degree.disciplineCivil engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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