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N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

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http://hdl.handle.net/10355/9375
dc.contributor.advisorMohan, Rajiv R.eng
dc.contributor.authorSharma, Ajayeng
dc.contributor.authorTandon, Ashisheng
dc.contributor.authorTovey, Jonathaneng
dc.contributor.authorGupta, Rangan, 1978-eng
dc.contributor.authorRobertson, John David, 1960-eng
dc.contributor.authorFortune, Jennifer A.eng
dc.contributor.authorKlibanov, Alexander M.eng
dc.contributor.authorCowden, John W.eng
dc.contributor.corporatenameUniversity of Missouri--Columbia. School of Medicineeng
dc.contributor.meetingnameHealth Sciences Research Day (2010 : University of Missouri)eng
dc.date.issued2010eng
dc.description.abstractRecently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo.eng
dc.description.sponsorship"National Eye Institute, NIH, Bethesda for RO1EY017294 (RRM), Diversity (RRM) and Veteran Health Affairs Merit (RRM) grants and Unrestricted grant from Research to Prevent Blindness, New York"eng
dc.format.extent2 files (1 page, 1 poster)eng
dc.identifier.urihttp://hdl.handle.net/10355/9375
dc.languageEnglisheng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Health Sciences Research Dayeng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.eng
dc.subjectnon-viral gene therapyeng
dc.subjectcorneal diseaseseng
dc.subject.lcshCornea -- Diseaseseng
dc.subject.lcshCornea -- Diseases -- Gene therapyeng
dc.subject.lcshNanoparticleseng
dc.titleN/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitroeng
dc.typeOthereng


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