N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

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N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/9375

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dc.contributor.advisor Mohan, Rajiv R. en_US
dc.contributor.author Sharma, Ajay
dc.contributor.author Tandon, Ashish
dc.contributor.author Tovey, Jonathan
dc.contributor.author Gupta, Rangan, 1978-
dc.contributor.author Robertson, John David, 1960-
dc.contributor.author Fortune, Jennifer A.
dc.contributor.author Klibanov, Alexander M.
dc.contributor.author Cowden, John W.
dc.date.accessioned 2010-12-17T20:36:45Z
dc.date.available 2010-12-17T20:36:45Z
dc.date.issued 2010-11-11
dc.identifier.uri http://hdl.handle.net/10355/9375
dc.description.abstract Recently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo. en_US
dc.description.sponsorship "National Eye Institute, NIH, Bethesda for RO1EY017294 (RRM), Diversity (RRM) and Veteran Health Affairs Merit (RRM) grants and Unrestricted grant from Research to Prevent Blindness, New York"
dc.language.iso en_US en_US
dc.relation.ispartof 2010 Health Sciences Research Day (MU) en_US
dc.subject non-viral gene therapy en_US
dc.subject corneal diseases en_US
dc.subject.lcsh Cornea -- Diseases
dc.subject.lcsh Cornea -- Diseases -- Gene therapy
dc.subject.lcsh Nanoparticles
dc.title N/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitro en_US
dc.type Other en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Health Sciences Research Day.


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