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dc.contributor.advisorMohan, Rajiv R.en_US
dc.contributor.authorSharma, Ajay
dc.contributor.authorTandon, Ashish
dc.contributor.authorTovey, Jonathan
dc.contributor.authorGupta, Rangan, 1978-
dc.contributor.authorRobertson, John David, 1960-
dc.contributor.authorFortune, Jennifer A.
dc.contributor.authorKlibanov, Alexander M.
dc.contributor.authorCowden, John W.
dc.date.issued2010-11eng
dc.description.abstractRecently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo.en_US
dc.description.sponsorship"National Eye Institute, NIH, Bethesda for RO1EY017294 (RRM), Diversity (RRM) and Veteran Health Affairs Merit (RRM) grants and Unrestricted grant from Research to Prevent Blindness, New York"
dc.identifier.urihttp://hdl.handle.net/10355/9375
dc.relation.ispartof2010 Health Sciences Research Day (MU)en_US
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Health Sciences Research Day.
dc.subjectnon-viral gene therapyen_US
dc.subjectcorneal diseasesen_US
dc.subject.lcshCornea -- Diseases
dc.subject.lcshCornea -- Diseases -- Gene therapy
dc.subject.lcshNanoparticles
dc.titleN/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitroen_US
dc.typeOtheren_US


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