[-] Show simple item record

dc.contributor.advisorMohan, Rajiv R.eng
dc.contributor.authorSharma, Ajayeng
dc.contributor.authorTandon, Ashisheng
dc.contributor.authorTovey, Jonathaneng
dc.contributor.authorGupta, Rangan, 1978-eng
dc.contributor.authorRobertson, John David, 1960-eng
dc.contributor.authorFortune, Jennifer A.eng
dc.contributor.authorKlibanov, Alexander M.eng
dc.contributor.authorCowden, John W.eng
dc.date.issued2010-11eng
dc.description.abstractRecently, we discovered that polyethylenimine-conjugated gold nanoparticles (PEI2- GNP) could be used as gene therapy vector for the cornea. It was hypothesized that DNA concentration, incubation timing and PEI monomer amount in transfection solution affect gene transfer efficiency and toxicity. The aims of this study were to test whether molar ratio of PEI2 nitrogen (N) and phosphate (P) of DNA in PEI2-GNP transfection solution regulates transgene delivery in human corneal fibroblasts in vitro, and examine PEI2-GNP toxicity, uptake and clearance for the cornea in vivo.eng
dc.description.sponsorship"National Eye Institute, NIH, Bethesda for RO1EY017294 (RRM), Diversity (RRM) and Veteran Health Affairs Merit (RRM) grants and Unrestricted grant from Research to Prevent Blindness, New York"eng
dc.identifier.urihttp://hdl.handle.net/10355/9375eng
dc.relation.ispartof2010 Health Sciences Research Day (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Health Sciences Research Day.eng
dc.subjectnon-viral gene therapyeng
dc.subjectcorneal diseaseseng
dc.subject.lcshCornea -- Diseaseseng
dc.subject.lcshCornea -- Diseases -- Gene therapyeng
dc.subject.lcshNanoparticleseng
dc.titleN/P ratio in the PEI2-GNP-DNA complex affects transgene delivery in the human cornea in vitroeng
dc.typeOthereng


Files in this item

[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record