Inhibition of β-glucosidase by D-Gluconic acid δ-lactone in water deficient maize [abstract]

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Inhibition of β-glucosidase by D-Gluconic acid δ-lactone in water deficient maize [abstract]

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dc.contributor.author Zelle, Karen en
dc.contributor.author Maltman, Rachel en
dc.contributor.author Bondra, Mary en
dc.contributor.author Sharp, Bob (Robert E.) en
dc.date 2007 en
dc.date.accessioned 2009-06-25T15:13:40Z en
dc.date.available 2009-06-25T15:13:40Z en
dc.date.issued 2007 en
dc.identifier.uri http://hdl.handle.net/10355/1850 en
dc.description Abstract only available en
dc.description.abstract The effects of β-glucosidase inhibitor D-Gluconic acid δ-lactone were measured under water deficit and well watered conditions in maize. Kinematic analysis had previously shown root growth maintenance in the apical 3mm of seedlings under water deficits (Zhu, et al., 2006). Distinct region-specific responses of cell wall proteins to water stress were analyzed and showed up-regulation of β-glucosidase. ß-glucosidase, a common hydrolase, has been found to cleave inactive ABA-conjugates in barley leaves, hence freeing ABA to its biologically active state (Dietz et al., 2000). Furthermore, Sauter and Hartung (2000) found that when lateral water flow increased in root systems, the ABA-conjugate concentration in the xylem decreased while the ABA concentration increased. This implies that something in the medium caused cleavage of the ABA-conjugate. The medium used to perform this experiment contained ß-glucosidases. D-Gluconic acid δ-lactone has been used as a ß-glucosidase inhibitor in maize coleoptiles (Jabben, et al., 2006). A hydroponic growth system using polyethylene glycol to impose water deficit was used to incorporate this inhibitor. Primary root elongation under water deficit conditions and exposed to D-Gluconic acid δ-lactone was inhibited by 28%. When inhibitor was added to well-watered roots there was no effect. These results suggest that ABA is present in a conjugated form and ß-glucosidase frees ABA to its active form under water deficit conditions. Experiments to add back and quantify ABA are in progress. en
dc.description.sponsorship MU Monsanto Undergraduate Research Fellowships en
dc.language en_US en
dc.language.iso en_US en
dc.publisher University of Missouri--Columbia. Office of Undergraduate Research en
dc.relation.ispartof 2007 Undergraduate Research and Creative Achievements Forum (MU) en
dc.source.uri http://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid= en
dc.subject root growth maintenance en
dc.subject cell wall proteins en
dc.subject ABA-conjugate en
dc.title Inhibition of β-glucosidase by D-Gluconic acid δ-lactone in water deficient maize [abstract] en
dc.type Presentation en
dc.contributor.meetingname Undergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia) en
dc.contributor.corporatename University of Missouri-Columbia. Office of Undergraduate Research en
dc.relation.ispartofcommunity University of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forum


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