dc.contributor.advisor | Lee, James C. | eng |
dc.contributor.author | Hicks, Jacob | eng |
dc.date.issued | 2010 | eng |
dc.date.submitted | 2010 Spring | eng |
dc.description | Title from PDF of title page (University of Missouri--Columbia, viewed on June 14, 2010). | eng |
dc.description | The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. | eng |
dc.description | Thesis advisor: Dr. James Lee. | eng |
dc.description | M.S. University of Missouri--Columbia 2010. | eng |
dc.description.abstract | Oligomeric amyloid-[beta] peptide (A[beta]) is known to induce cytotoxic effects and damage cell functions in Alzheimer's disease. However, mechanisms underlying the effects of A[beta] on cell membranes have yet to be fully elucidated. In this study, A[beta] 1-42 (A[beta][subscript 42]) was shown to cause a temporal biphasic change in membranes of astrocytic DITNC cells using fluorescence microscopy of Laurdan. A[beta][subscript 42] made astrocyte cell membranes become more molecularly-disordered after 30 minutes to 1 hour, transitioning to more molecularly-ordered after 3 hours. However, A[beta][subscript 42] caused artificial vesicle membranes made of rat whole brain lipid extract to become more disordered only. The trend for more molecularly-ordered membranes in astrocytes was abrogated by either an NADPH oxidase inhibitor, apocynin, or an inhibitor of cytosolic phospholipase A[subscript 2] (cPLA[subscript 2]), but not by an inhibitor of calcium-independent PLA2 (iPLA[subscript 2]). Apocynin also suppressed the increased production of superoxide anions (O[subscript 2]-) and phosphorylation of cPLA[subscript 2] induced by A[beta][subscript 42]. In addition, hydrolyzed products of cPLA[subscript 2], arachidonic acid (AA), but not lysophosphatidylcholine (LPC) caused astrocyte membranes to become more molecularly-ordered. These results suggest (1) a direct interaction of A[beta][subscript 42] with cell membranes making them more molecularly-disordered, and (2) A[beta][subscript 42] indirectly makes membranes become more molecularly-ordered by triggering the signaling pathway involving NADPH oxidase and cPLA[subscript 2]. | eng |
dc.description.bibref | Includes bibliographical references. | eng |
dc.format.extent | vi, 38 pages | eng |
dc.identifier.merlin | b79329330 | eng |
dc.identifier.oclc | 648195908 | eng |
dc.identifier.uri | http://hdl.handle.net/10355/8096 | |
dc.identifier.uri | https://doi.org/10.32469/10355/8096 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | OpenAccess. | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License. | |
dc.source | Submitted by University of Missouri--Columbia Graduate School. | eng |
dc.subject.lcsh | Phospholipase A2 -- Inhibitors | eng |
dc.subject.lcsh | Alzheimer's disease -- Alternative treatment | eng |
dc.subject.lcsh | Amyloid beta-protein | eng |
dc.title | Amyloid- [beta] peptide induces temporal membrane biphasic changes in astrocytes through cytosolic phospholipase A₂ | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Biological engineering (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Masters | eng |
thesis.degree.name | M.S. | eng |