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dc.contributor.advisorHaidekker, Mark A., 1963-en
dc.contributor.authorNipper, Matthew Edwarden_US
dc.date.issued2007eng
dc.date.submitted2007 Summeren
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on May 12, 2009)en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2007.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.en_US
dc.description.abstractMembrane viscosity can be defined as the "thickness" of a cell membrane. Membrane viscosity conditions are a useful indicator of cell health. Many diseases have been reported to induce changes in afflicted cell membrane viscosity. Characterizing membrane viscosity will provide researchers and clinicians with a valuable tool in diagnosing the onset and progression of diseases. Molecular rotors are fluorescent molecules that have been shown to exhibit viscosity sensitive DLPC liposomes were formed with the molecular rotor FCVJ incorporated into the membrane. A cuvette with liposomes was excited at 460 nm under fluorescent spectroscopy and intensity values were recorded. The procedure was repeated for a 2% cyclohexane/sucrose solution. Peak emissions were compared and the cyclohexane fluidized the membrane resulting in a statistically significant reduction in intensity. To achieve a converse effect, a 20% (v/v) Cholesterol/DLPC mixture was used to produce liposomes in the presence of sucrose solution. Intensity values were compared to those of the control group for the cyclohexane experiments. The intensity values for the cholesterol group were higher than control. The liposomes used in this experiment are a suitable model for the mammalian cell membrane in both size and physical similarities. This previously undocumented method for characterizing membrane conditions provides a qualitative method of measuring changes in membrane viscosity.en_US
dc.identifier.merlin.b67519660en_US
dc.identifier.oclc320835608en_US
dc.identifier.otherNipperM-040109-T7708en_US
dc.identifier.urihttp://hdl.handle.net/10355/4973
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2007 Freely available theses (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2007 Theses
dc.subject.lcshViscosityen_US
dc.subject.lcshMembranes (Biology) -- Fluidityen_US
dc.subject.lcshCell interactionen_US
dc.titleCharacterization of membrane viscosity changes with the novel molecular rotor FCVJen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological engineeringeng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMastersen_US
thesis.degree.nameM.S.en_US


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