Characterization of membrane viscosity changes with the novel molecular rotor FCVJ

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Characterization of membrane viscosity changes with the novel molecular rotor FCVJ

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/4973

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dc.contributor.advisor Haidekker, Mark A., 1963- en
dc.contributor.author Nipper, Matthew Edward en_US
dc.date.accessioned 2010-01-12T19:07:32Z
dc.date.available 2010-01-12T19:07:32Z
dc.date.issued 2007 en_US
dc.date.submitted 2007 Summer en
dc.identifier.other NipperM-040109-T7708 en_US
dc.identifier.uri http://hdl.handle.net/10355/4973
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Title from title screen of research.pdf file (viewed on May 12, 2009) en_US
dc.description Includes bibliographical references. en_US
dc.description Thesis (M.S.) University of Missouri-Columbia 2007. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering. en_US
dc.description.abstract Membrane viscosity can be defined as the "thickness" of a cell membrane. Membrane viscosity conditions are a useful indicator of cell health. Many diseases have been reported to induce changes in afflicted cell membrane viscosity. Characterizing membrane viscosity will provide researchers and clinicians with a valuable tool in diagnosing the onset and progression of diseases. Molecular rotors are fluorescent molecules that have been shown to exhibit viscosity sensitive DLPC liposomes were formed with the molecular rotor FCVJ incorporated into the membrane. A cuvette with liposomes was excited at 460 nm under fluorescent spectroscopy and intensity values were recorded. The procedure was repeated for a 2% cyclohexane/sucrose solution. Peak emissions were compared and the cyclohexane fluidized the membrane resulting in a statistically significant reduction in intensity. To achieve a converse effect, a 20% (v/v) Cholesterol/DLPC mixture was used to produce liposomes in the presence of sucrose solution. Intensity values were compared to those of the control group for the cyclohexane experiments. The intensity values for the cholesterol group were higher than control. The liposomes used in this experiment are a suitable model for the mammalian cell membrane in both size and physical similarities. This previously undocumented method for characterizing membrane conditions provides a qualitative method of measuring changes in membrane viscosity. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Viscosity en_US
dc.subject.lcsh Membranes (Biology) -- Fluidity en_US
dc.subject.lcsh Cell interaction en_US
dc.title Characterization of membrane viscosity changes with the novel molecular rotor FCVJ en_US
dc.type Thesis en_US
thesis.degree.discipline Biological engineering en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name M.S. en_US
thesis.degree.level Masters en_US
dc.identifier.merlin .b67519660 en_US
dc.identifier.oclc 320835608 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2007 Theses
dc.relation.ispartofcollection 2007 Freely available theses (MU)


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