Near infrared fluorescent sensors and their use in vivo
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In an effort to design near-infrared (NIR), water soluble glucose sensors, several pH sensitive NIR cyanine derivates were designed and synthesized to provide insight into the viability of the cyanine platform as the fluorophore core for performing minimally invasive long term glucose monitoring in vivo. Many previous efforts to build effective fluorescent sensors for glucose have provided guidance towards the architecture of binding groups and fluorescent response required to achieve this goal, but have not provided appropriate solubility, or excitation and emission characteristics for in vivo sensing. In an effort to address the aqueous solubility of the highly rigid cyanine platform, a tetra sulfonated core was chosen for this work. Though fully water soluble, pH sensitive derivates still showed some aggregation characteristics. Simple sugarbinding boronic acid derivatives showed appropriate fluorescent responses, but poor binding. Efforts to improve binding through synthesis of bis-boronic acid compounds proved elusive.