Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2019 Dissertations (MU)
    • 2019 MU dissertations - Freely available online
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2019 Dissertations (MU)
    • 2019 MU dissertations - Freely available online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    The design of fluorescence correlation spectroscopy for single molecule techniques

    Norman, Zenia
    View/Open
    [PDF] NormanZenia.pdf (3.008Mb)
    Date
    2019
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    The ribosome is responsible for protein synthesis- a critical process in creating essential proteins for cell survival. Though bulk techniques yield valuable results about the structure of the ribosome, bulk techniques are not ideal in examining ribosomal dynamics and understanding kinetics involved in protein synthesis. On the contrary, the single molecule spectroscopy techniques are ideal in investigating the mechanisms of ribosome dynamics in real-time under equilibrium conditions. The latest advances in single molecule biophysics have opened numerous opportunities in the biological world to study the dynamics of molecules in real-time. Single molecule techniques such as Fluorescence Correlation Spectroscopy (FCS) have opened opportunities to study the ribosome as well as other ribosomal proteins. FCS is used to investigate diffusion coefficients, concentration, and kinetics of biological samples. In this thesis, we address the process of assembling an FCS system, as well as the design of a high-speed correlator. The high-speed correlator allows the software to handle the high data counts that can occur in single molecule experiments. A field-programmable gate arrays (FPGA) dual correlator and a multi-tau correlator are designed to handle the noise and high count rates that can dominate the signal. Also addressed in this thesis are ideal experimental conditions for successfully obtaining and troubleshooting results received from FCS curves.
    URI
    https://hdl.handle.net/10355/72229
    Degree
    Ph. D.
    Thesis Department
    Biological engineering (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2019 MU dissertations - Freely available online
    • Biological Engineering electronic theses and dissertations - CAFNR (MU)
    • Biological Engineering electronic theses and dissertations - Engineering (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems